FastQCFastQC Report
Fri 29 Sept 2023
SRR16312994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16312994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54047919
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length18-75
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC30341725.613855364163049No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC27252675.042316245330371No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG23800154.4035275437709265No Hit
CTCGGTTGGCCTCGGATAGCCGGTC17216643.1854399426553313No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC16763863.1016661344537613No Hit
TCGCTTCTGGCGCCAAGCGCCCGG13480222.4941237793077655No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG11546022.136256161869988No Hit
TCGGTTGGCCTCGGATAGCCGGTC11469482.1220946545601507No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC9387831.7369456907304792No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT8285191.5329341357250037No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC7283201.347544944329864No Hit
CGGTTGGCCTCGGATAGCCGGTC6589211.219142220813349No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTC6544331.210838478351035No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC5556841.0281320914501815No Hit
CTCGCTTCTGGCGCCAAGCGCCCG5259300.9730809432274348No Hit
CGCTGAGAAGACGGTCGAACTTGACTATC5057460.9357363046669752No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC4926640.9115318574985283No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC4858750.8989707818352821No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA4168640.771285939797238No Hit
GCTGAGAAGACGGTCGAACTTGACTATCT4138440.7656983056091392No Hit
CCTCGGTTGGCCTCGGATAGCCGGTC3996950.739519684374897No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC3435510.6356414943561471No Hit
CTAAACCATTCGTAGACGACCTGCTT2713210.5020008263407885No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC2633160.48718989532233425No Hit
GCTGAGAAGACGGTCGAACTTGACTATC2427080.4490607677235455No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG2368470.43821668693664223No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC2354750.43567819882204906No Hit
CGCTGAGAAGACGGTCGAACT2263040.41870992294818976No Hit
CGCTTCTGGCGCCAAGCGCCCGGC2226230.4118992999526957No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC2171430.40176014917429104No Hit
AAGTCCTTCTGATCGAGGC1946480.3601396753129385No Hit
TCGCTTCTGGCGCCAAGCGCCCG1895750.35075356000293No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC1823230.3373358371115084No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG1765140.3265879672444003No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA1759770.3255944044765165No Hit
TTTTTCACTGACCCGGTGAGGCGGGG1727860.3196903843790914No Hit
AAGTCCTTCTGATCGAGGCC1647430.304809145380787No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAG1583650.2930085060259212No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1532770.2835946375659718No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT1443960.2671629225909697No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1396280.2583411213297593No Hit
TTTTTTCACTGACCCGGTGAGGCGGGG1390890.2573438581418833No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1373960.25421145261855504No Hit
CGGCCGAGGTGGGATCCCGAGGC1288460.23839215715224854No Hit
GCTGAGAAGACGGTCGAACT1279470.2367288183657913No Hit
GCCGTGATCGTATAGTGGTTAGTACT1269680.2349174627796493No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG1198080.22166995920786517No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG1151350.21302392789628033No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC1144240.21170842858908223No Hit
CGCTGAGAAGACGGTCGAACTTG1106870.20479419383380884No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG968880.17926314609818741No Hit
CGGTTGGCCTCGGATAGCCGGTCCCC949590.17569409101578914No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG939470.1738216784997772No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC938860.1737088156900176No Hit
CAGTACACGGAGTCAAGACCCGCAACGCCT905340.16750691178322705No Hit
CTGTACACGGAGTCAAGACCCGCAACGCCT881480.16309231073262967No Hit
AACCTCGGGCCGATCGCAC842430.15586724069801836No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG832210.15397632608204584No Hit
AAGTACACGGAGTCAAGACCCGCAACGCCT785870.14540245296030732No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG779090.14414801058297916No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTC751840.1391061883437177No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC735670.13611439878008993No Hit
CAGTACACGGAGTCGACCCGCAACGCCT733000.13562039271114212No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCC732380.13550567969138644No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG730780.13520964609201697No Hit
TAGTACACGGAGTCAAGACCCGCAACGCCT713970.1320994430886414No Hit
CTCGGTTGGCCTCGGATAGCCGGTCC713090.13193662460898817No Hit
ACACGGAGTCAAGACCCGCAACGCCT702750.13002350747306293No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA691440.12793091996751993No Hit
TTTTCACTGACCCGGTGAGGCGGGG686560.12702801748944303No Hit
CTGAGAAGACGGTCGAACTTGACTATCT674210.12474300814430987No Hit
TTGTACACGGAGTCAAGACCCGCAACGCCT668760.12373464369645759No Hit
ATGTACACGGAGTCAAGACCCGCAACGCCT666760.12336460169724574No Hit
TAGTACACGGAGTCGACCCGCAACGCCT659890.12209350742995304No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCT648850.12005087559430364No Hit
CGCTGAGAAGACGGTCGAACTTGACT646740.11966048128513514No Hit
AAACCATTCGTAGACGACCTGCTT615580.11389522693741455No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT606260.11217083122108734No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAG578710.10707350268194414No Hit
AAGTACACGGAGTCGACCCGCAACGCCT574850.10635932162346529No Hit
TTGTACACGGAGTCGACCCGCAACGCCT567010.10490875698655484No Hit
GCTGAGAAGACGGTCGAACTTG562930.10415387130816268No Hit
GAGTACACGGAGTCAAGACCCGCAACGCCT560190.10364691376924244No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC547780.10135080316413293No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC546520.10111767670462947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)