FastQCFastQC Report
Fri 29 Sept 2023
SRR16312995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16312995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29535492
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA23917738.097962275353328No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA19857526.7232738157874605No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC6845232.317628567013544RNA PCR Primer, Index 1 (100% over 24bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG6060682.0519989983576368No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT6028742.04118489036851RNA PCR Primer, Index 1 (100% over 25bp)
CTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACT4895071.6573517718953186RNA PCR Primer, Index 1 (100% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC4468751.5130101777210958No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG4195811.4205993250425624Illumina Small RNA Adapter 2 (100% over 21bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG3719741.2594135895890952Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA3247351.0994738127267356No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC2620160.8871225168688573No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAA2400940.8128999510148671RNA PCR Primer, Index 1 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACT2376770.8047165762466391RNA PCR Primer, Index 1 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA2330620.789091307502174RNA PCR Primer, Index 1 (100% over 23bp)
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG2160440.7314724941775136Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTC2033250.6884090503723452RNA PCR Primer, Index 1 (100% over 26bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT1969580.6668519352919532No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1834250.6210324852553666No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC1739280.5888779506364749No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAA1709460.5787816231400513No Hit
TCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTC1633320.5530024690294646RNA PCR Primer, Index 1 (100% over 26bp)
CTCGGTTGGCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGA1579830.5348920546168657RNA PCR Primer, Index 1 (100% over 22bp)
GCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGA1389160.4703358251150853RNA PCR Primer, Index 1 (100% over 22bp)
CCTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAAC1371620.46439720726507616RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG1343790.45497464541982235No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG1339350.45347136929359433No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGCCAAGGA1302550.44101178338251484RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1175750.3980803841019476No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1061710.3594692108057655RNA PCR Primer, Index 1 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG982790.33274881623776575No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCA854080.2891707373623571No Hit
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC852190.2885308292815979RNA PCR Primer, Index 1 (100% over 42bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTAGTGGAATTCTCGGGTGCCA764620.25888175487308623No Hit
CGCTGAGAAGACGGTCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCC708990.24004678845370173RNA PCR Primer, Index 1 (100% over 27bp)
TCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAAC699280.23675921836683814RNA PCR Primer, Index 1 (100% over 24bp)
CGCTGAGAAGACGGTCGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAG687930.23291638412524157RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTTCTGGCGCCAAGCGCCCGTGGAATTCTCGGGTGCCAAGGAACTC674550.22838624120431109RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG631280.21373607048766957No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGG625460.211765559889776Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGTTGGCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAA572940.19398356391015933RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC569870.1929441364985557No Hit
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT566620.1918437654602131RNA PCR Primer, Index 1 (100% over 43bp)
CGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCC542600.1837111770476009RNA PCR Primer, Index 1 (100% over 27bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA532070.1801459748833708No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCTGGAATTCTCGGGTGCCAAGGAA530850.17973291252436221RNA PCR Primer, Index 1 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC528200.17883568690848284No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGCCAAGGAA458540.15525050336049928RNA PCR Primer, Index 1 (100% over 23bp)
CGCTGAGAAGACGGTCGAACTTGACTTGGAATTCTCGGGTGCCAAGGAAC434930.14725673098657033RNA PCR Primer, Index 1 (100% over 24bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG422240.14296020530147255No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA405590.1373229198281173No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA401680.13599908882506512No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG393440.13320922502323645Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC392750.13297560778740372RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG391470.13254223088614878No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA386880.13098816840430488No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA359590.12174843743926798RNA PCR Primer, Index 1 (100% over 23bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGA333340.1128608252065007RNA PCR Primer, Index 1 (100% over 22bp)
CTCGGTTGGCCTCGGATAGCCGGTCCTGGAATTCTCGGGTGCCAAGGAAC326220.11045016619327012RNA PCR Primer, Index 1 (100% over 24bp)
GGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT324110.10973577145760768RNA PCR Primer, Index 1 (100% over 35bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCA311030.10530720124790878No Hit
CTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGGA307750.10419667293844301RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCTCGGGTG300130.1016167260731597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)