FastQCFastQC Report
Fri 29 Sept 2023
SRR16312996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16312996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49196905
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length18-75
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC31502146.403276791497352No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC29447805.98570174282305No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG15593783.1696668723367862No Hit
CTCGGTTGGCCTCGGATAGCCGGTC15418003.133936982417898No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG14199572.886273028760651No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC13226912.6885654697180645No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT9705961.9728802045575835No Hit
TCGGTTGGCCTCGGATAGCCGGTC8959351.8211206578950443No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC7659011.556807282897166No Hit
CGCTGAGAAGACGGTCGAACTTGACTATC6898221.402165441098378No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTC6811131.3844631079942935No Hit
TCGCTTCTGGCGCCAAGCGCCCGG6570131.3354762865672138No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC6296481.2798528687932706No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC5834241.1858957387664935No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC4833040.9823870017839538No Hit
CCTCGGTTGGCCTCGGATAGCCGGTC4004230.8139190869832157No Hit
GCTGAGAAGACGGTCGAACTTGACTATCT3895790.7918770499892218No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC3582800.7282571942279702No Hit
CGGTTGGCCTCGGATAGCCGGTC3279080.6665216033406979No Hit
CTAAACCATTCGTAGACGACCTGCTT3220840.654683460270519No Hit
GCTGAGAAGACGGTCGAACTTGACTATC2765940.5622182940166663No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA2762150.5614479203519002No Hit
CTCGCTTCTGGCGCCAAGCGCCCG2615300.5315984816524536No Hit
AAGTCCTTCTGATCGAGGCC2576720.5237565249277368No Hit
AAGTCCTTCTGATCGAGGC2572140.522825572055803No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC2505290.5092373188923165No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC2418230.49154108373280797No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG2412890.4904556495982827No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC2384460.48467683078844087No Hit
CGCTGAGAAGACGGTCGAACT2367500.4812294594548174No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC2298210.46714524013248393No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG2017990.41018637249639994No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC1918000.38986192322464186No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1801470.3661754738433241No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG1712500.3480910028791445No Hit
AACCTCGGGCCGATCGCAC1712460.3480828722863765No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC1593630.32392891382089994No Hit
CGCTGAGAAGACGGTCGAACTTG1381780.28086726187348576No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1340640.2725049472116183No Hit
TTTTTCACTGACCCGGTGAGGCGGGG1149410.23363461583609785No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC1148720.23349436311085017No Hit
CGCTTCTGGCGCCAAGCGCCCGGC1091300.22182289719241485No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA1072760.2180543674444561No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG1068800.21724943876042607No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT1048070.21303575905842045No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAG1041600.21172063567819968No Hit
CGGTTGGCCTCGGATAGCCGGTCCCC1022170.2077712002411534No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1015880.20649266452838852No Hit
TTTTTTCACTGACCCGGTGAGGCGGGG1005640.20441123277978565No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCC989640.20115899567259363No Hit
AAACCATTCGTAGACGACCTGCTT922840.187580905750067No Hit
GCTGAGAAGACGGTCGAACT909520.18487341835832966No Hit
CTCGGTTGGCCTCGGATAGCCGGTCC908490.18466405559455418No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG876990.1782612137897699No Hit
CTGTACACGGAGTCAAGACCCGCAACGCCT819260.16652673577738275No Hit
CAGTACACGGAGTCAAGACCCGCAACGCCT812490.16515063295140212No Hit
CGCTGAGAAGACGGTCGAACTTGACT755980.15366413801843837No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT750570.15256447534656906No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC739080.15022896257396678No Hit
AAGTACACGGAGTCAAGACCCGCAACGCCT719690.1462876577296885No Hit
GCCTCGGTTGGCCTCGGAT705180.14333828520310374No Hit
TCGCTTCTGGCGCCAAGCGCCCG696700.14161459953629196No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG660700.13429706604510996No Hit
TAGTACACGGAGTCAAGACCCGCAACGCCT644330.13096962095481413No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG643990.1309005109162863No Hit
TTGTACACGGAGTCAAGACCCGCAACGCCT634370.1289451033555871No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG632530.12857109608826003No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC624440.12692668370093607No Hit
ATGTACACGGAGTCAAGACCCGCAACGCCT623150.1266644720841687No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTC620500.12612582031329003No Hit
CAGTACACGGAGTCGACCCGCAACGCCT595690.12108282014895043No Hit
CGGTACACGGAGTCAAGACCCGCAACGCCT577660.11741795545878345No Hit
ACACGGAGTCAAGACCCGCAACGCCT572370.11634268456521808No Hit
GCTGAGAAGACGGTCGAACTTG558960.11361690333975279No Hit
GAGTACACGGAGTCAAGACCCGCAACGCCT546090.11100088511665519No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCT544150.11060655136740818No Hit
TAGTACACGGAGTCGACCCGCAACGCCT542570.11028539295307298No Hit
CTGAGAAGACGGTCGAACTTGACTATCT529450.10761855852517552No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG525640.10684411956402542No Hit
GCCGTGATCGTATAGTGGTTAGTACT519600.10561640005606043No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC519390.10557371444402855No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC502760.10219342050074086No Hit
TTGTACACGGAGTCGACCCGCAACGCCT500650.10176453173222991No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGG493050.10021971910631369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)