FastQCFastQC Report
Mon 25 Sept 2023
SRR1635380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1635380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68230346
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC35557645.211411356465934No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA15633172.2912341672721404RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC13822772.025897684880566No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA12902131.8909665209670783No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG7873001.1538853987344575No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA7221671.0584249418872946No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA7094481.039783676313176RNA PCR Primer, Index 1 (100% over 23bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC6996651.0254454813991418No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA6683110.9794923214957755RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA5422730.7947680640517344No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA4756380.6971062406747872No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC4262050.6246560731203092No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA3874330.5678309179320299RNA PCR Primer, Index 1 (100% over 23bp)
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA3685520.5401584802164128No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC3680220.5393816997498444RNA PCR Primer, Index 1 (100% over 24bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA3476720.5095562610806634RNA PCR Primer, Index 1 (100% over 22bp)
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG3358480.4922267285585801Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA3237980.4745659651205638RNA PCR Primer, Index 4 (100% over 36bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA3146450.46115111302528056RNA PCR Primer, Index 1 (100% over 23bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG2990920.43835627039030406Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC2793150.40937063399913No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC2778130.4071692674693457RNA PCR Primer, Index 1 (100% over 24bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG2307540.33819849015568526Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA2295430.3364236200707527RNA PCR Primer, Index 1 (100% over 23bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG2206110.32333267077379324RNA PCR Primer, Index 4 (100% over 35bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG2050610.30054222500938216Illumina Small RNA Adapter 2 (100% over 21bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC1990360.29171184329037403No Hit
TGAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAG1979420.29010845115749523No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1907880.2796233804823443RNA PCR Primer, Index 4 (100% over 50bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA1860760.2727173624474951RNA PCR Primer, Index 1 (100% over 28bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCA1839590.2696146374517872No Hit
TCTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGC1832450.2685681822572027No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG1732110.25386211583918983Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG1727160.2531366321958854No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1702760.24956051080262734No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC1353110.1983149843619436No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCAA1346570.19735646657866868No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG1341840.19666322665284447Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACT1314030.19258732763864336RNA PCR Primer, Index 1 (100% over 25bp)
CGGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAG1300930.1906673608250499No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG1294820.18977186485321357Illumina Small RNA Adapter 2 (100% over 21bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1282390.1879500948155825RNA PCR Primer, Index 3 (100% over 34bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1240930.18187361969408744RNA PCR Primer, Index 1 (100% over 22bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG1221540.17903177568526474No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA1221080.1789643570032607No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCA1195840.17526512323416915No Hit
AGGGCACCACAAGGCGTGGAGCTTGCGGCTTAATTTGTGGAATTCTCGGG1187020.17397244328791767No Hit
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA1157120.1695902289576547RNA PCR Primer, Index 1 (100% over 23bp)
TAGGTGTAAGTTTCCAGCAGTGTCGTTGGAATTCTCGGGTGCCAAGGAAC1119720.16410879698602143RNA PCR Primer, Index 1 (100% over 24bp)
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1110490.16275602647537504No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG1090060.15976175762028233No Hit
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC1063260.15583388658178576RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1038580.15221672772991654No Hit
AGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCCA1027060.15052832943277175No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGTGGAATTCTCGGGTGCCA1019500.14942031805027048No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTTGGAATTCTCGGGTGC1010060.1480367694456657No Hit
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA971320.14235894392210763No Hit
TGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAAC948450.1390070629276891RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG934870.13701674618504794No Hit
CGGCACTGTTGGTTCGCTTGCTCCTTTGGAATTCTCGGGTGCCAAGGAAC927880.1359922753432908RNA PCR Primer, Index 1 (100% over 24bp)
GTGGTGTCTCGTGCTCTTTGAACGGCTGGAATTCTCGGGTGCCAAGGAAC879640.128922107473997RNA PCR Primer, Index 1 (100% over 24bp)
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA852530.12494880210632377RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC785860.11517749008630265No Hit
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT763840.11195018709123943RNA PCR Primer, Index 1 (100% over 25bp)
TCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGAA748760.1097400268203242RNA PCR Primer, Index 1 (100% over 23bp)
CCACGCGGCCCGGGTTCGATTCCCGGCATGGGATGGAATTCTCGGGTGCC720840.105648005947383No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAGG719230.10541204056036885Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT710730.10416626056681583RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)