FastQCFastQC Report
Mon 25 Sept 2023
SRR1635381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1635381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67084608
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC34798145.1872018093926995No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA15404192.296233138904233RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC13504142.0130012535811495No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA12618461.8809769299091679No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG7626991.136921005784218No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA7013761.0455095750130938No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA6892351.0274115338051912RNA PCR Primer, Index 1 (100% over 23bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC6829721.0180755621319275No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA6545520.9757111497170855RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA5294850.7892794126485766No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA4614650.6878850659751936No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC4155830.619490837600184No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA3807900.5676264814724713RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC3576560.5331416708882013RNA PCR Primer, Index 1 (100% over 24bp)
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA3569500.5320892685249051No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA3382370.5041946432779334RNA PCR Primer, Index 1 (100% over 22bp)
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG3286650.4899260945223083Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA3162370.4714002353565217RNA PCR Primer, Index 4 (100% over 36bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA3077110.4586909116320692RNA PCR Primer, Index 1 (100% over 23bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG2906980.4333303997244793Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC2726680.40645389177797686RNA PCR Primer, Index 1 (100% over 24bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC2714490.4046367834481496No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA2255720.3362500083476675RNA PCR Primer, Index 1 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG2245300.3346967459361169Illumina Small RNA Adapter 2 (100% over 21bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG2169700.32342739485039546RNA PCR Primer, Index 4 (100% over 35bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG2001630.2983739578533425Illumina Small RNA Adapter 2 (100% over 21bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC1948030.29038404755976216No Hit
TGAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAG1941590.2894240658006081No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCA1901230.28340778260193455No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1862250.2775972097802226RNA PCR Primer, Index 4 (100% over 50bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA1809140.2696803415770127RNA PCR Primer, Index 1 (100% over 28bp)
TCTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGC1785360.2661355642116892No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG1698020.25311618426688876Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG1690670.2520205529113325No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1647680.24561222747250755No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCAA1366910.20375910969025862No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC1319140.1966382512065957No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG1315820.1961433537779635Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACT1266040.18872287365829132RNA PCR Primer, Index 1 (100% over 25bp)
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG1259650.1877703451736649Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAG1258320.18757208807123088No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1238900.18467723624471355RNA PCR Primer, Index 3 (100% over 34bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1202460.17924528976900334RNA PCR Primer, Index 1 (100% over 22bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG1196440.17834791551588108No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA1188210.17712110652863916No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCA1180540.17597777421610633No Hit
AGGGCACCACAAGGCGTGGAGCTTGCGGCTTAATTTGTGGAATTCTCGGG1151020.17157736093501508No Hit
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA1141400.17014335091590607RNA PCR Primer, Index 1 (100% over 23bp)
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1089090.16234573510513767No Hit
TAGGTGTAAGTTTCCAGCAGTGTCGTTGGAATTCTCGGGTGCCAAGGAAC1080110.16100712699998188RNA PCR Primer, Index 1 (100% over 24bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG1059530.15793935920442437No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1016760.15156382817352082No Hit
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC1011930.15084384185415528RNA PCR Primer, Index 1 (100% over 24bp)
AGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCCA1004760.14977504228689836No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGTGGAATTCTCGGGTGCCA1002180.14939045332127454No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTTGGAATTCTCGGGTGC984040.14668640532266358No Hit
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA951810.14188202456217677No Hit
TGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAAC921960.13743241966920342RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG919250.13702845218980783No Hit
CGGCACTGTTGGTTCGCTTGCTCCTTTGGAATTCTCGGGTGCCAAGGAAC903270.13464638565078893RNA PCR Primer, Index 1 (100% over 24bp)
GTGGTGTCTCGTGCTCTTTGAACGGCTGGAATTCTCGGGTGCCAAGGAAC845290.12600356850859143RNA PCR Primer, Index 1 (100% over 24bp)
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA836250.12465601647400251RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC760720.11339709997261965No Hit
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT743210.11078696323305638RNA PCR Primer, Index 1 (100% over 25bp)
TCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGAA730920.10895494835417388RNA PCR Primer, Index 1 (100% over 23bp)
CCACGCGGCCCGGGTTCGATTCCCGGCATGGGATGGAATTCTCGGGTGCC707400.10544892801639387No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAGG702150.10466633419099654Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT687260.10244674903667916RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)