FastQCFastQC Report
Mon 25 Sept 2023
SRR1635385.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1635385.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65196636
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA37092245.689287404337855RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC32946965.0534754584577035No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC14945812.2924204248820446No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG11160091.711758563739393Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA8894081.3641930850542656No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA7594331.1648346396277256RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA6987331.0717316764625708RNA PCR Primer, Index 1 (100% over 23bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC6303460.9668382276656114No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA6245490.9579466646101188RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC5229520.8021150048293904RNA PCR Primer, Index 1 (100% over 24bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA4703420.7214206573480263RNA PCR Primer, Index 1 (100% over 23bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA4575390.7017831410810828No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG4507770.6914114403080551Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC4477170.6867179466130737No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC3947670.6055020998322674RNA PCR Primer, Index 1 (100% over 24bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG3828640.5872450228873772No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA3767760.5779071177844206RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA3686260.565406472812493RNA PCR Primer, Index 1 (100% over 23bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG3504300.5374970573635118No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG3497350.5364310514425928No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA3195290.490100440151544No Hit
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG3087070.47350142421458674Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC2936260.4503698626413792No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA2699050.41398608357645933No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC2470230.37888918072398703No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA2401110.3682874067306172No Hit
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC2129030.32655519220347506RNA PCR Primer, Index 1 (100% over 24bp)
TCTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGC2029500.3112890671230338No Hit
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG1957010.3001703952946284Illumina Small RNA Adapter 2 (100% over 21bp)
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT1835550.2815405997327837RNA PCR Primer, Index 1 (100% over 25bp)
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1760440.27002006667951395No Hit
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT1732700.2657652459246517RNA PCR Primer, Index 1 (100% over 25bp)
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA1727960.26503821454837023No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC1701250.26094137740480966No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTTGGAATTCTCGGGTGC1653520.25362044753352No Hit
TGAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAG1560050.2392838182632613No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA1514760.232337140830395RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC1500580.23016218198742647No Hit
CTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACT1332240.20434183137915274RNA PCR Primer, Index 1 (100% over 25bp)
CGGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAG1259800.1932308286580921No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG1180120.181009339193513Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG1152250.17673457875955442No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG1123510.17232637585779734Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCACTGTTGGTTCGCTTGCTCCTTGGAATTCTCGGGTGCCAAGGAACT1074060.16474162869384856RNA PCR Primer, Index 1 (100% over 25bp)
TGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAAC1057570.162212357091553RNA PCR Primer, Index 1 (100% over 24bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1010230.1549512462575523RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1000470.1534542365038589No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG981130.1504878257829131No Hit
TAGGTGTAAGTTTCCAGCAGTGTCGTTGGAATTCTCGGGTGCCAAGGAAC973090.14925463332187877RNA PCR Primer, Index 1 (100% over 24bp)
AGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCCA941130.14435253990712035No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG878570.13475695279738054Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA853730.13094694026851322RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG800860.1228376261621842No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAAC796060.12210139185708907RNA PCR Primer, Index 1 (100% over 24bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG794740.12189892742318792No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA789870.12115195636781015RNA PCR Primer, Index 1 (100% over 22bp)
GTGGTGTCTCGTGCTCTTTGAACGGCTGGAATTCTCGGGTGCCAAGGAAC778350.11938499403558182RNA PCR Primer, Index 1 (100% over 24bp)
AGGGCACCACAAGGCGTGGAGCTTGCGGCTTAATTTGTGGAATTCTCGGG756220.1159906471247995No Hit
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA756070.11596763980276528No Hit
ATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG696340.10680612416873779RNA PCR Primer, Index 7 (100% over 36bp)
TTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACT667200.10233656840822278RNA PCR Primer, Index 1 (100% over 25bp)
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC667070.10231662872912646No Hit
CGGCACTGTTGGTTCGCTTGCTCCTTTGGAATTCTCGGGTGCCAAGGAAC666930.10229515522856118RNA PCR Primer, Index 1 (100% over 24bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA666130.10217244951104532RNA PCR Primer, Index 1 (100% over 23bp)
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA652900.10014320370762687RNA PCR Primer, Index 7 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)