FastQCFastQC Report
Mon 25 Sept 2023
SRR1635386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1635386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62835601
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA60930509.69681184397361RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG23471733.7354190341873235Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC18527862.9486246180728024No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA10452211.6634216644160051RNA PCR Primer, Index 1 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA10056781.6004907791046672RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA8247511.31255369070155No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG7202811.1462944390394227Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA6696411.0657031831365789RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC6251150.994842080049493RNA PCR Primer, Index 1 (100% over 24bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC5764760.9174353246020516No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG5380950.8563537094202378No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA5188830.8257786855575711RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA4892280.7785841023466936RNA PCR Primer, Index 1 (100% over 22bp)
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG4873090.775530101160328Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC4673150.7437105598783085RNA PCR Primer, Index 1 (100% over 24bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA3945050.6278367577004635No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC3796770.6042386703677745No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC3179190.5059536233289151RNA PCR Primer, Index 12 (100% over 50bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC2993350.4763780328925317No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC2879630.45828001231340176No Hit
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC2852260.453924201345667RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA2777620.4420455849543RNA PCR Primer, Index 1 (100% over 23bp)
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT2758540.43900908976743935RNA PCR Primer, Index 1 (100% over 25bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC2326980.3703282793459714No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA2306180.3670180539850331RNA PCR Primer, Index 1 (100% over 22bp)
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA2242660.3569091349981677No Hit
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT2239780.3564507961020378RNA PCR Primer, Index 1 (100% over 25bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG1953310.3108603990276149No Hit
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG1949530.31025882922644443Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC1897230.30193552218908515No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC1886930.3002963240536205No Hit
TGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAAC1587620.25266249940061847RNA PCR Primer, Index 1 (100% over 24bp)
TCTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGC1574200.25052676746101304No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1573350.2503914938284747No Hit
TAGGTGTAAGTTTCCAGCAGTGTCGTTGGAATTCTCGGGTGCCAAGGAAC1490740.23724448820024815RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1474600.23467588063652003No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG1468460.2336987275732431Illumina Small RNA Adapter 2 (100% over 21bp)
TGAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAG1448120.23046170911932554No Hit
CGGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAG1404700.22355161367836682No Hit
CGGCACTGTTGGTTCGCTTGCTCCTTGGAATTCTCGGGTGCCAAGGAACT1356540.2158871688041943RNA PCR Primer, Index 1 (100% over 25bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1301850.2071835041412272RNA PCR Primer, Index 1 (100% over 22bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA1253410.19947449854104204RNA PCR Primer, Index 1 (100% over 22bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG1249190.19880290474185167No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA1239500.19726078533091454RNA PCR Primer, Index 1 (100% over 22bp)
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG1151120.18319551045592766Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG1144490.1821403761221286No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG949770.15115157408934465No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA936170.14898719596873117No Hit
TTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACT910590.14491625535657723RNA PCR Primer, Index 1 (100% over 25bp)
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTTGGAATTCTCGGGTGC859170.13673299631525765No Hit
AGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCCA833960.1327209395196204No Hit
CTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACT813070.12939639106817807RNA PCR Primer, Index 1 (100% over 25bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG806040.12827759855436094Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAAC791170.1259111057121901RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGAA776350.1235525701425216RNA PCR Primer, Index 1 (100% over 23bp)
ATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGGA763160.12145344165642659RNA PCR Primer, Index 1 (100% over 22bp)
GTGGTGTCTCGTGCTCTTTGAACGGCTGGAATTCTCGGGTGCCAAGGAAC746180.11875115191466061RNA PCR Primer, Index 1 (100% over 24bp)
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA745890.11870499973414751No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGTGGAATTCTCGGGTGCCA743470.11831986774503836No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG738720.11756392685732409No Hit
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG731820.11646582325201282Illumina Small RNA Adapter 2 (100% over 21bp)
TCTATTAATTAATCTGACGTGATATTAGTGGAATTCTCGGGTGCCAAGGA714500.1137094240572315RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG713370.11352958969868053No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAA706760.11247763827388235No Hit
ATAGGTGTAAGTTTCCAGCAGTGTCGTTGGAATTCTCGGGTGCCAAGGAA706700.11246808954687965RNA PCR Primer, Index 1 (100% over 23bp)
CGGCACTGTTGGTTCGCTTGCTCCTTTGGAATTCTCGGGTGCCAAGGAAC702460.11179331283868837RNA PCR Primer, Index 1 (100% over 24bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG666080.10600360136604726Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA650260.1034859203463336RNA PCR Primer, Index 1 (100% over 23bp)
TCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGAA646750.10292731981667526RNA PCR Primer, Index 1 (100% over 23bp)
CATCCGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG639900.10183717348386624Illumina Small RNA Adapter 2 (100% over 21bp)
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA638160.10156026040078775No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)