FastQCFastQC Report
Mon 25 Sept 2023
SRR1635387.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1635387.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61703784
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA59898119.707364138316056RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG22850693.7032882780738374Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC18089052.9315949245511423No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA10213701.6552793585560328RNA PCR Primer, Index 1 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA9867191.5991223488011692RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA8041841.3032977037518478No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG7077941.1470836213221542Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA6497421.0530018710035676RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC6086660.9864322097328747RNA PCR Primer, Index 1 (100% over 24bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC5618960.9106345892822391No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG5261270.8526656971313137No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA5094610.8256560083900202RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA4767550.772651155397536RNA PCR Primer, Index 1 (100% over 22bp)
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG4761720.7717063186918974Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC4571500.7408783876204416RNA PCR Primer, Index 1 (100% over 24bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA3847780.6235889844292207No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC3696820.5991237101439354No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC3110290.5040679514890043RNA PCR Primer, Index 12 (100% over 50bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC2906910.4711072500837226No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC2820030.457027076329711No Hit
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC2759350.4471929954895473RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA2702730.43801689698641494RNA PCR Primer, Index 1 (100% over 23bp)
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT2691940.4362682197902158RNA PCR Primer, Index 1 (100% over 25bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC2277430.36909081621315154No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA2243010.3635125521637376RNA PCR Primer, Index 1 (100% over 22bp)
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA2194630.35567186608847196No Hit
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT2169770.3516429397587675RNA PCR Primer, Index 1 (100% over 25bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG1910530.309629308957778Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG1897010.30743819536254047No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC1842020.2985262621819109No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC1832710.29701744061595964No Hit
TGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAAC1545640.2504935515786196RNA PCR Primer, Index 1 (100% over 24bp)
TCTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGC1545070.2504011747480511No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1532200.24831540315258463No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1440860.23351242121552868No Hit
TAGGTGTAAGTTTCCAGCAGTGTCGTTGGAATTCTCGGGTGCCAAGGAAC1434580.23249465543312545RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG1430810.2318836718344535Illumina Small RNA Adapter 2 (100% over 21bp)
TGAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAG1405820.22783367710479474No Hit
CGGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAG1357270.2199654400449736No Hit
CGGCACTGTTGGTTCGCTTGCTCCTTGGAATTCTCGGGTGCCAAGGAACT1314030.21295776609097425RNA PCR Primer, Index 1 (100% over 25bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1266210.20520783620012673RNA PCR Primer, Index 1 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA1218430.1974643888938805RNA PCR Primer, Index 1 (100% over 22bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA1214000.19674644264928715RNA PCR Primer, Index 1 (100% over 22bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG1205500.19536889342151204No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG1127600.18274405991049106No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG1125610.18242155132657667Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG925720.1500264554277579No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA912290.14784992764787325No Hit
TTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACT894050.144893869069683RNA PCR Primer, Index 1 (100% over 25bp)
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTTGGAATTCTCGGGTGC833470.13507599469102252No Hit
AGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCCA820140.13291567337264115No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG789420.12793704839884698Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACT782400.1267993548013198RNA PCR Primer, Index 1 (100% over 25bp)
TGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAAC764500.12389839819224052RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGAA754210.12223075330355752RNA PCR Primer, Index 1 (100% over 23bp)
ATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGGA743110.12043183607669831RNA PCR Primer, Index 1 (100% over 22bp)
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA732500.11871233051120496No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGTGGAATTCTCGGGTGCCA725950.11765080728274298No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCTGGAATTCTCGGGTGCCAAGGAAC723940.11732505740652793RNA PCR Primer, Index 1 (100% over 24bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG721910.11699606623801223No Hit
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG715090.11589078556349154Illumina Small RNA Adapter 2 (100% over 21bp)
TCTATTAATTAATCTGACGTGATATTAGTGGAATTCTCGGGTGCCAAGGA696680.11290717600074575RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG695230.11267218230894883No Hit
ATAGGTGTAAGTTTCCAGCAGTGTCGTTGGAATTCTCGGGTGCCAAGGAA690540.11191209926444705RNA PCR Primer, Index 1 (100% over 23bp)
CAACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAA685820.11114715428149431No Hit
CGGCACTGTTGGTTCGCTTGCTCCTTTGGAATTCTCGGGTGCCAAGGAAC676480.10963347077709204RNA PCR Primer, Index 1 (100% over 24bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG643400.10427237331182151Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA634850.10288672085329482RNA PCR Primer, Index 1 (100% over 23bp)
TCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGAA625050.10129848762597769RNA PCR Primer, Index 1 (100% over 23bp)
CATCCGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG623240.10100515067276912Illumina Small RNA Adapter 2 (100% over 21bp)
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA622430.10087387833459291No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)