FastQCFastQC Report
Mon 3 Jul 2023
SRR1640905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153324075
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC50197093.2739209416394655No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT49168873.20685906632732No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG44628752.910746404307347No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC38666042.521850531301102No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG33794962.204152218104039TruSeq Adapter, Index 1 (100% over 49bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA17680251.1531294090637756No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC15048840.9815053506763369No Hit
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGGAAGAGCACACGTCT7926560.5169807807417067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGGAAGAGCACACGTCT7572360.49387938586944025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7010050.45720478013645277Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC7001760.45666409531575525No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGAGCACACGTCT6831360.44555038078657905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA6490110.4232936021299981Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG5988110.3905524947729181No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT5389660.35152079019553845No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC5311660.34643352650260567No Hit
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACGTCTG5244290.3420395655411585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA5207920.3396674657910051Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC5163490.3367696821259153No Hit
AGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGT5156250.33629747970108415No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC5081720.33143653402115747Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT4986630.32523463780883727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGATCGGAAGAGCACA4751750.30991545196017No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT4716140.307592920420358TruSeq Adapter, Index 1 (100% over 35bp)
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGTCT4588680.2992798097754707Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCA4317350.2815833064702983No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTC4301670.28056063602535997No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT4149380.2706280797715558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGA4127160.2691788618323639Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC4087780.2666104458807268TruSeq Adapter, Index 1 (100% over 36bp)
AGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGTCTG4049810.2641339920035389Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA3960050.2582797254769024No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG3765120.24556613173762828Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC3683880.2402675509374506No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC3502100.2284116176797414No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG3453460.22523925221789204Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT3372560.21996284666970922Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG3372550.21996219445641527No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGGTAGATCGGAAGAGCACACGTCTG3178220.20728773351477908Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCGGAAGAGCACACGTC3140780.20484584694217134No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3102510.20234982666616444Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAGATCGGAAGAGCAC3004670.19596857179800367No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT2810760.18332150381471402No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT2700940.17615889742038227No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA2650350.1728593503662096Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGCTAATACATGCCGACGGGCGCTGACCAGATCGGAAGAGCACACGTC2582180.16841321234124518No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACG2561830.1670859582880249No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2545310.1660085019263935No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA2495940.16278852489408463Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2470760.1611462518198789No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGGAAGAGCACACGTCT2466880.1608931930618202Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTAGATCGGAAGAGCACACGTC2430770.1585380508573099No Hit
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGA2321650.15142109939355577Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGTGTAGAATAAGTGGGAGGCCCCAGATCGGAAGAGCACACGTCTGA2304390.1502953792481709Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGT2295460.1497129527766595No Hit
TGATCCTTCGATGTCGGCTCTTCCTATCAGATCGGAAGAGCACACGTCTG2257170.14721562807406469Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2209220.14408826532949898No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG2181740.1422959831976811Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTGATCATCGACACTTCGAACGCACTTAGATCGGAAGAGCACACGTCTG2149450.14018998647146577Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCGGCATTGGCAATTTTTGACAGTCTCTAGATCGGAAGAGCACACGTC2106440.13738481709411912No Hit
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA2098840.13688913499070512TruSeq Adapter, Index 1 (100% over 37bp)
AAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTAGATCGGAAGAGCACA2084480.13595255670057033No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCTG2078780.13558079512300986Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCTAATACATGCCGACGGGCGCTGACAGATCGGAAGAGCACACGTCT2031750.13251343600148902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGAAGAGCACACGTCTG2028860.1323249463595329Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATCTCGCGCCGGGCCGTACCCATATCCGCAGATCGGAAGAGCACACGT2019000.1316818640516827No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCT2009160.1310400861704204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATACCGTCGTAGTTCCGACCATAAACAGATCGGAAGAGCACACGTCTG2005020.1307700698667186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT1999450.13040678706197967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG1998540.13034743565222878Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACTCTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCACAC1964920.12815469455791595No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT1920740.12527321622517534No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG1907800.12442925222278367Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGT1865450.12166712892283876No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG1854120.12092817126077558No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGAGATCGGAAGAGCACACGTCTG1850010.12066011159695567Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1832480.11951678169263372No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTC1799410.11735991232948903Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGAGATCGGAAGAGCACACGTCT1781620.11619962487952398Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA1779220.11604309368897218Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1743320.11370164796363519Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG1731940.11295942923510219No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACAC1630130.106319245689237No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGAGATCGGAAGAGCACACGTC1589520.10367060750244213No Hit
GCGCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCT1558250.10163113653221126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCCCGAGTGTTACAGCCCCCCCGGCAGATCGGAAGAGCACACGTCTGA1553650.10133111841698704Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1551420.10118567485243267Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTCCCCGCCCCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1534080.10005473700069607Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)