FastQCFastQC Report
Fri 30 Jun 2023
SRR1640906.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640906.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98126929
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT31854373.2462414063727603No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG27932242.846541747984389No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT20304822.0692403407427538No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC19806602.0184673261302204No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC17203861.7532251518846576No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG14736781.5018079287898636No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT10320351.0517347383815507TruSeq Adapter, Index 1 (100% over 35bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG9132830.9307159709441228No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA7895520.8046231631278301No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC7634860.7780596088969625No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT7428520.7570317420205823No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG6588610.6714375011165385No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA4926620.5020660536517962Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA4921670.5015616049698244No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGAT4905620.4999259683343397No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAC4897060.49905362879541454No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACA4734170.48245370034967666No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT4283560.43653256487829145Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGA3975930.40518235315404605No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG3962220.4037851831682209No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATC3960110.4035701555482288No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCA3801390.38739518690124297No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCAC3615130.36841364922364994No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAC3600350.36690743679545906No Hit
GGGATGCGTGCATTTATCAGATCAAAACCAACAGAT3499520.3566319700069285No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC3489580.35561899628999905No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG3367020.34312905074202416No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT3300320.3363317321384836No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATC3155940.3216181360368467No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA3140500.32004466378439295TruSeq Adapter, Index 1 (100% over 36bp)
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCAC3016080.3073651678225862No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGG2878370.2933313035813033No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCA2852560.2907010368173246No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT2797000.2850389825202825No Hit
GCATTTATCAGATCAAAACCAACAGATCGGAAGAGC2667770.2718693051119535No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAAC2659650.2710418054558703Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCA2596730.26462970220947196No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGAT2514000.2561987851469396No Hit
TTTATCAGATCAGATCGGAAGAGCACACGTCTGAAC2306150.23501703594535198Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA2282070.2325630714480018No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGA2258340.2301447750392759No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2246540.2289422509085146Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGA2148800.21898168238812404No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC2144170.2185098445300372No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACAC2078740.2118419501337905No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCG2025630.2064295724571183No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACG2008360.20466960705557186No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGG1999840.20380134386963236No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA1996020.20341205215950459No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCA1991770.2029789396547812No Hit
ATTTATCAGATCAAAACCAACCAGATCGGAAGAGCA1962310.1999767056808636No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACA1948750.19859482201873452No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGG1925540.19622951819882184No Hit
GGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCAC1909800.19462547329897587No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATC1888070.19241099453953153No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA1840540.18756726810435492No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCG1742930.17761994773116765No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1682570.17146873107584973No Hit
CGTGCATTTATCAGATCAGATCGGAAGAGCACACGT1677360.17093778609947125No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGG1652830.16843796263103272No Hit
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAAC1594170.1624599909776041Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCG1589340.16196777135458912No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA1530880.1560101814660887No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA1524870.15539770942999753Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1493260.15217637148310226No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAC1476710.15048978043529723No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGAT1463490.14914254577354602No Hit
CATTTATCAGATCAGATCGGAAGAGCACACGTCTGA1452790.1480521213498896Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAAC1451450.14791556352487092Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCTACCATCTGTGGGATTATGACTGAACAGATCG1448830.1476485624043121No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGAT1431090.1458406998551845No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1419370.1446463284303945No Hit
CATTTATCAGATCAAAACCAACCAGATCGGAAGAGC1405700.1432532347975549No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATC1380860.14072181959347776No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCA1365460.13915242369400962No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT1336060.13615630424957048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCG1299190.13239892588506463No Hit
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACT1273510.12978190726828923Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCG1258550.12825735125166304No Hit
CGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACA1253520.12774474986371986No Hit
CGTGCATTTATCAGATAGATCGGAAGAGCACACGTC1235960.12595523090302765No Hit
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC1232270.12557918734010315Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGA1229660.1253132053077907No Hit
GCATTTATCAGATCAAAACCAACCAGATCGGAAGAG1222150.12454787003473838No Hit
CATTTATCAGATAGATCGGAAGAGCACACGTCTGAA1215380.12385794729192023Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGG1205180.1228184772805842No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCG1192410.12151710158992135No Hit
CGTGCATTTATCAGATCAAAACCAACAGATCGGAAG1173560.11959612024544251No Hit
ATTTATCAGATCAGATCGGAAGAGCACACGTCTGAA1133420.1155055000243613Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGTACGTAGCAGAAGATCGGAAGAGCACACGTC1129300.11508563566684127No Hit
CAAAACCAACAGATCGGAAGAGCACACGTCTGAACT1121960.11433762489397788Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATCAGATCAAAACCAACAGATCGGAAGAGCACACG1121100.1142499833047868No Hit
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGG1116700.11380158447636733No Hit
TATCAGATCAAAACCAACCAGATCGGAAGAGCACAC1114580.11358553776812887No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGG1098710.11196824472107957No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCG1092190.11130379918442164No Hit
CAAAACCAACCAGATCGGAAGAGCACACGTCTGAAC1081560.11022050837849007Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGA1047010.10669955848715088No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCT1046950.1066934439576724Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCCAGA1027990.1047612526424831No Hit
TCGTACGTAGCAGAAGATCGGAAGAGCACACGTCTG1022860.10423846037207585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGA1018430.10378700427891716No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG1015690.10350777409940139Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCTACCATCTGTGGGATTATGACTGAACGAGATC1001410.10205251608353094No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAC990870.10097839707181705No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)