FastQCFastQC Report
Mon 3 Jul 2023
SRR1640911.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640911.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206690852
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACN18147000.8779778990896027No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCN16974640.8212574400728678No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCN16807750.8131830624027812No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGN15921220.7702914689228724Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAN15762090.7625925311875922No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAN15619790.7557078530016412No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCN13492590.6527908646871319No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCN9604600.46468432961900025No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCN9570860.46305194000554994No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAN9231040.446610960798594No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCN8809010.42619254382869354No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGN8337000.40335602274260307No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAN7930230.38367590646924227No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATN7724780.373735940669498No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTN7072460.342175763056993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAN6189460.29945495604227323Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTN5981010.2893698459378357Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCACN5976150.2891347121642326No Hit
TTTCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCN5723610.27691646459515296No Hit
TACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCAN5524170.26726727121914423No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAAN5502380.2662130397527221Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACN5439990.26319452202945104No Hit
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAN5253070.2541510642183622Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCN5038640.24377663313323608No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAN5033540.24352988781525756No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACN5010230.24240211656779082No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTN4925330.23829453274497123No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATN4694700.22713632241450143No Hit
GCGCGACCGCTCGAGATCGGAAGAGCACACGTCTGN4682370.2265397793222121Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGN4531130.21922257110827528No Hit
TGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCN4515370.2184600796942866No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAN4497210.21758147283654333No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAN4305400.2083014298088045No Hit
GCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGN4292150.20766037579640922Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAN4230810.2046926585797808No Hit
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTN4122280.19944182145032718No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGN4083510.1975660732193411No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCN3696100.17882262152560097No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN3500790.16937324347571994No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN3479030.16832046345234478Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCN3472370.1679982430959257No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGN3232700.1564026645939802No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGN3151970.15249683135468423No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGACCCGGN3065130.148295387548163No Hit
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACN2926970.14161100850268885Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACN2864640.13859539366551163No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAAN2782320.13461263394472822Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATN2729780.13207067335520006No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAN2677190.129526293693927No Hit
TGGATACCGCAGCTAGGAATAATGGAATAGATCGGN2652350.12832449885106673No Hit
TTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCAN2602430.12590929762097067No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGN2498230.12086795210462435No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTN2405580.11638541216134712Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTAGGAATAATGGAATAGATCGGAAGAGCACACGN2365880.11446466919590617No Hit
CGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAN2257070.10920028526468117Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTN2246990.1087126004009118Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTN2163670.10468145924523065No Hit
ACTACTCTGAGATCGGAAGAGCACACGTCTGAACTN2130070.10305584303266599Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCN2124680.10279506709856708No Hit
CGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCN2118890.10251493859050909No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGN2095690.1013924893008811No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)