FastQCFastQC Report
Thu 6 Jul 2023
SRR1640912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215972689
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCN51502712.38468624150899No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGN49448522.2895728264975204No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAN47952372.220297863680347No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAN44683512.068942615239652No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACN36183181.6753590543107977No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCN28295681.3101508404148265No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCN26849571.2431928372202654No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATN23298681.07877899320872No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCN21367810.9893755594254791No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCN16138050.7472264236150711No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTN14791680.6848865969344855Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATN13184930.6104906162463902No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGN12991860.6015510600046287No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAN12689970.5875729037202477No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACN12425440.5753245957872016No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAN11725920.5429353153073906No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAN11721690.5427394572097957No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTN10733760.49699617343746644No Hit
TCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACN9933930.45996232421776256No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCN9427070.43649361609791315No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAN9308260.4309924575694847No Hit
TACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCAN9074040.4201475678251151No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATN8710480.4033139579051127No Hit
TTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCN7680050.35560283272668797No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACN7575310.35075314545905384No Hit
GATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTN7427030.3438874625485633Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGN7011500.3246475298550365No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAAN6835250.3164867757885813Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCN6769510.313442872399482No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCN6740830.31211492671649793No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAN6546610.30312212300139485No Hit
TCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCACN6513250.30157748325298667No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGN6507730.3013218953809479No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGN6114980.28313672568108833Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACN6100980.2824884955708451No Hit
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTN5952220.27560058762800327Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACAN5713940.2645677111516633No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN5438460.25181239466810545No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN5371640.24871848495621593Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAN5238880.2425714114250807No Hit
TTTCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCN5185540.2401016547050539No Hit
TGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCN5123580.23723277344572025No Hit
ATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGN4987350.23092503145154616Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACN4976830.23043793282584912No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGN4835890.2239121077017289No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAN4788330.2217099774129311No Hit
TCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACAN4733800.21918512113353372No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACN4627410.21425903531719234No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTN4578250.21198282158722392Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAAN4511540.20889400511191486Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AAATACCACTACTCTGAGATCGGAAGAGCACACGTN4475750.20723685113722876No Hit
TTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCAN4281080.19822321145429642No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCN4255160.19702305970733178No Hit
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTN4242800.19645076512428847No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGN4074330.18865024179052567No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAN4016780.1859855530159186No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN3983470.184443228375047Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAN3745960.17344600455477036No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAN3743170.173316821554229No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGAN3700500.17134110878250908No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGN3652550.16912092065492595No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAN3540820.16394758135367754No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCN3522150.16308312019951743No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATN3413910.15807137540432253No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGN3408280.1578106942957033Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGAN3370900.1560799199013538Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AAGCTACCATCTGTGGGATTATGACTGAACAGATCN3359950.15557291135084214No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCN3346040.15492884843416474No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACN3244470.15022593898435No Hit
GCAGCTAGGAATAATGGAATAGATCGGAAGAGCACN3193360.14785943606045485No Hit
CGTAGACGACCTGCTTAGATCGGAAGAGCACACGTN3174470.14698478843313378No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAN3133050.14506695334982841No Hit
TGGATACCGCAGCTAGGAATAATGGAATAGATCGGN2973940.13769981814691393No Hit
TAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACAN2963770.13722892527397296No Hit
GCTAGGAATAATGGAATAGATCGGAAGAGCACACGN2960510.13707798026258775No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAN2939160.1360894293444668Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATTAATGATGAGATATAACCTTGACTGAAGAGATCN2930620.13569400897721842No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGN2892340.13392156264721045No Hit
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAN2791430.1292492126168786Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AAGCTACCATCTGTGGGATTATGACTGAACGAGATN2742790.12699707600529064No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCN2723550.1261062226252135No Hit
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGN2720980.12598722609783314No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCAN2557520.11841867653923593No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCAGATCGN2516960.11654066130555979No Hit
TATCAGATCAAAACCAACAGATCGGAAGAGCACACN2475130.11460384234045445No Hit
ACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCACN2473300.1145191094046155No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGN2467210.11423712930665969No Hit
AAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGN2462090.1140000622949136No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGAN2457320.11377920103592357No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCN2454260.11363751645468469No Hit
ACTACTCTGAGATCGGAAGAGCACACGTCTGAACTN2437800.11287538305364156Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ACGGTCGAACTTGACTATCTAGAGATCGGAAGAGCN2437570.11286473355897328No Hit
AAATACCACTACTCTGATAGATCGGAAGAGCACACN2324770.10764185095644199No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCN2319390.10739274538550568No Hit
TGATCGAGGCCAGATCGGAAGAGCACACGTCTGAAN2294990.10626297290765316Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTN2204450.10207077618040862Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGTGTCGGCGCGGCGGATCTTTCCCGCCCAGATCGN2191900.1014896841887263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)