FastQCFastQC Report
Sun 2 Jul 2023
SRR1640935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161592574
Total Bases8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA59722413.6958635240255533Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA49892543.0875515356293537Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG26847561.6614352587761863Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG20303241.2564463513032473TruSeq Adapter, Index 1 (100% over 49bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC15479090.9579084989388188Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT13880310.8589695464594803No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC12372650.7656694669644906Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT9774160.6048644289805051Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC9245700.5721611934964289TruSeq Adapter, Index 1 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT9216500.5703541797657112Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT8927070.5524430844204511No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA8336010.5158659085410694Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC7983530.49405302498616055No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT7897460.4887266663627748TruSeq Adapter, Index 1 (100% over 35bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT7730310.47838275043505407Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA7493950.4637558406613413Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT7414480.4588379166483232TruSeq Adapter, Index 1 (100% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT7317590.45284197280006194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG7092150.4388908366544121Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG6904190.42725911402339567No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC6898120.42688347794992115No Hit
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTC6843120.4234798561968572No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6513280.4030680271235732Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5468570.33841716018460105Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA5428330.3359269467419957Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG5297500.32783065885193463Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT5204300.32206306708128807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5089420.3149538294996155Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC5079180.31432013701322686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC4986570.3085890568213859TruSeq Adapter, Index 1 (100% over 36bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC4534660.280623043977256TruSeq Adapter, Index 1 (100% over 36bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4394180.2719295751796119Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCC4353410.2694065632001134Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC4347760.2690569184200259No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT4331470.2680488275408002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT4312990.26690521063177075No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG4293240.2656830010022614No Hit
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC3928060.2430841902425541Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC3737890.23131570390109635No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG3737340.2312816676835657No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT3605220.2231055493923873Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC3495210.2162976870459406No Hit
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC3427340.21209761780265965TruSeq Adapter, Index 1 (100% over 38bp)
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC3414670.21131354711881747TruSeq Adapter, Index 1 (100% over 36bp)
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAG3193680.1976377949150064Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATACCACTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3167100.19599291734779842Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG3163460.1957676594717775No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCA3133790.19393156024607913Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT2993730.18526408274182204TruSeq Adapter, Index 1 (100% over 41bp)
GATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2966540.18358145591517097TruSeq Adapter, Index 1 (100% over 35bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT2958060.18305667932488037Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA2922430.18085175126921366TruSeq Adapter, Index 1 (100% over 40bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT2907760.17994391252162367TruSeq Adapter, Index 1 (100% over 48bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2894550.1791264244605696No Hit
GATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG2845550.17609410689874896TruSeq Adapter, Index 1 (100% over 39bp)
CTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA2822040.17463921330939378TruSeq Adapter, Index 1 (100% over 37bp)
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGA2773340.17162546095713532Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA2729610.1689192722432901TruSeq Adapter, Index 1 (100% over 37bp)
CTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2610850.16156992461794686Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2464530.1525150530741592No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2413370.149349066003491Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2332220.14432717681692478Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA2320010.14357157278774454TruSeq Adapter, Index 1 (100% over 40bp)
CGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG2263250.14005903513858253Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT2179900.1349010010818938TruSeq Adapter, Index 1 (100% over 41bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT2175160.13460767077081154No Hit
TGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2162380.13381679284346323TruSeq Adapter, Index 1 (100% over 38bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTAGATCGGAAGAGCACACGTC2159910.1336639392847347No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2098280.12985002640034685No Hit
CCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC2049890.12685545809796928Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGTCTGAAC1983390.12274016997835557Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAA1940550.12008905805287809Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1938100.11993744217478705TruSeq Adapter, Index 1 (100% over 36bp)
GCATGGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA1892590.11712109988420631Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC1884030.11659137257136581TruSeq Adapter, Index 1 (100% over 42bp)
GACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1880750.11638839294681946TruSeq Adapter, Index 1 (100% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG1801190.11146489937093272Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTACGTAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1770130.10954278134092969TruSeq Adapter, Index 1 (100% over 35bp)
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG1744950.10798454141834513TruSeq Adapter, Index 1 (100% over 39bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1737750.10753897638885311No Hit
GAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT1718280.10633409428826848TruSeq Adapter, Index 1 (100% over 43bp)
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA1713060.10601105964188676Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1690710.10462795152950531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1685520.10430677340407982TruSeq Adapter, Index 1 (100% over 37bp)
ATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1682650.10412916623260175TruSeq Adapter, Index 1 (100% over 36bp)
GATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT1673550.10356602154254935TruSeq Adapter, Index 1 (100% over 41bp)
GTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1672750.10351651431705024Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG1672390.10349423606557565Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAATACCACTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1647830.1019743642427529Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1639100.10143411664449384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAG1621220.10032763015458866Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGGAATAATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1620210.10026512728239603TruSeq Adapter, Index 1 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)