FastQCFastQC Report
Sun 2 Jul 2023
SRR1640936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180235842
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC65459083.631856975484377No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC51961052.8829476658699216TruSeq Adapter, Index 1 (100% over 36bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA51692742.8680610596864526No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA48153772.6717088824097486Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG41788352.3185371753083386No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT40644292.2550614544248084No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA30564701.6958169729636794TruSeq Adapter, Index 1 (100% over 37bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA29283271.6247195715933127Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG28073371.5575908592032432Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA24142721.3395071552971134Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC21690791.2034670662231544Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCA19974161.1082235241534255Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC18253121.0127353026708195TruSeq Adapter, Index 1 (100% over 36bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG14658540.8132977235460193Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG13933300.7730593341140216No Hit
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC13833680.7675321315945582TruSeq Adapter, Index 1 (100% over 38bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13793140.7652828564476094Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAG12778880.7090088108002403Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA11850260.6574863172886556Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT11810960.6553058408881848Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT11304220.6271904563799247TruSeq Adapter, Index 1 (100% over 35bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA10985940.6095313716791136Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA10810940.5998218711681109Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTC10731810.5954315124513359Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC10491660.5821073036072371Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT9994430.5545195610981749TruSeq Adapter, Index 1 (100% over 35bp)
TCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG9659870.5359572154355402Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG8201910.4550654247782747TruSeq Adapter, Index 1 (100% over 39bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACAC8159230.4526974163107913No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC7942590.4406776095067706TruSeq Adapter, Index 1 (100% over 38bp)
CGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA7827710.43430373854274784TruSeq Adapter, Index 1 (100% over 37bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG7637690.4237608854736007Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC7618460.4226939500745917No Hit
TTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC7404750.4108367080505553Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC7208290.3999365453626033No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC7039220.3905560582117734Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT6712740.3724420140584468TruSeq Adapter, Index 1 (100% over 35bp)
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6482280.3596554341283572Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGAGATCGGAAG6440130.3573168315767072No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG6338100.35165591536449226No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCA6256460.34712629466895933Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA6220030.3451050540768689Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC6215030.34482763977655456Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAG6106090.3387833370013052Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA6058340.33613403043330303Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5309760.294600671047438Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGTAGCAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5091950.2825159492971437Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTG5083020.28202048735678226Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC5072920.28146011047014724TruSeq Adapter, Index 1 (100% over 38bp)
TCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT5050290.2802045333469244Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG4551470.25252857309036236Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCTGAGCGTCGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4235870.2350181824545198Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3984110.221049817605091TruSeq Adapter, Index 1 (100% over 49bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGTC3927390.21790282978232486No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCC3925060.21777355471837836Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCA3881000.2153289799040082Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC3871270.21478913167559646TruSeq Adapter, Index 1 (100% over 38bp)
GATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT3836410.2128549991738047TruSeq Adapter, Index 1 (100% over 35bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC3782670.20987335027402598TruSeq Adapter, Index 1 (100% over 36bp)
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC3648650.20243753736839978TruSeq Adapter, Index 1 (100% over 38bp)
ATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAAC3625460.20115088984354176Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGA3578010.1985182281335585Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3505390.19448906283579268Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT3469450.19249500884513304Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC3436120.19064576511923748No Hit
TACGCCTGTCTGAGCGTCGCTGCAGAGCAGCTCAGATCGGAAGAGCACAC3271580.18151661532449245No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC3263810.18108551350180394Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACG3244910.18003688744661564No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC3108620.17247512844864674Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC3091290.17151361048375718TruSeq Adapter, Index 1 (100% over 36bp)
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCA3050410.16924547116438693Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC2968540.16470308941103956Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACCGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG2908990.1613990850942955TruSeq Adapter, Index 1 (100% over 39bp)
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2892320.16047418581704742TruSeq Adapter, Index 1 (100% over 36bp)
TCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA2887490.16020620360294374TruSeq Adapter, Index 1 (100% over 37bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCAC2881670.15988329335737783No Hit
CACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2840440.1575957350369856Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA2823940.15668026784594818TruSeq Adapter, Index 1 (100% over 37bp)
GCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2767980.15357544699682985TruSeq Adapter, Index 1 (100% over 38bp)
GTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2760820.15317818971877967TruSeq Adapter, Index 1 (100% over 35bp)
TCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCT2677460.14855313850393864Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCC2638130.14637099761766584Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC2579310.1431074957887677Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAA2546730.14129986420791932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCC2545640.1412393878904508Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACG2490450.13817728884358085No Hit
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTC2446970.13576489408804715Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2424890.13453983253785892TruSeq Adapter, Index 1 (100% over 35bp)
GCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2421400.1343461973562395Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA2411670.13380634912782774No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGT2407130.13355445694314233No Hit
TACGCCTGTCTGAGCGTCGCTCAGATCGGAAGAGCACACGTCTGAACTCC2316090.1285032973630184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGT2229810.12371623619679376No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2225380.12347044712671523No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2210490.12264430734037907No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT2183870.1211673536055054TruSeq Adapter, Index 1 (100% over 48bp)
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGT2182600.12109689037322555Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAG2167380.12025244124306861Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACT2084310.1156434800576458Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCCA2063400.11448333345373113Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT2054660.11399841325678162Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACT2047550.11360393012173461Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2047490.11360060115013083Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2031040.11268790810209658TruSeq Adapter, Index 1 (100% over 35bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACAC1998560.11088582480725448No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA1993770.11062006190755333Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1967000.10913478574367022No Hit
GTGTCGGCGCGGCGGATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTG1933100.10725391678753884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAA1874350.1039942987588451Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT1871100.10381397946364077TruSeq Adapter, Index 1 (100% over 41bp)
GGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGA1868460.10366750471307477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT1868350.10366140159846786TruSeq Adapter, Index 1 (100% over 41bp)
CGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG1829060.1014814800265976Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1814010.10064646298265137Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)