FastQCFastQC Report
Sun 2 Jul 2023
SRR1640938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192043753
Total Bases9.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA118287386.159397436895539No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC57442992.991140774050588No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA46304952.4111666886659937Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG41159562.1432386816560496No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT40498102.1087954889113214No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAG34736071.8087581323199824Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA30432121.584645140735195Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG26126211.3604300890745455Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA22355671.1640925388497276Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC21542271.1217376073670045TruSeq Adapter, Index 1 (100% over 36bp)
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCA19537621.0173525404911243Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA18759650.9768425010940085TruSeq Adapter, Index 1 (100% over 37bp)
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCA17881090.9310945928035472Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC17631780.9181126552968375No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG17577350.9152784053329763Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC16737430.87154253853808Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACAC14796190.770459323402204No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG13376150.6965157570108516No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC12492540.6505048878106439No Hit
TATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC11875740.618387206794485Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTC11740310.6113351679812257Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGAGC11171260.5817038995275207No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC10490590.5462604138964103Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT9944930.5178470970623033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG9929160.5170259300233525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA9148340.47636748694449854Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG8681360.4520511531557082Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA8575120.4465190804722505Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC7700970.40100080735247867TruSeq Adapter, Index 1 (100% over 36bp)
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC7506210.3908593683857032Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGAGATCGGAAG7476630.38931909438366374No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAG7430180.3869003747286692Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC7164720.3730774830254437Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG7097930.36959962972604476No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCAC7059380.36759227466253486No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACG6938830.3613150592823501No Hit
TGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTG6651610.3463590924511874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGA6457600.3362567070848694Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT6404530.33349327431650433TruSeq Adapter, Index 1 (100% over 35bp)
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC5865760.3054387298919325TruSeq Adapter, Index 1 (100% over 38bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC5786590.3013162318276502No Hit
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCCA5652270.2943219923430678Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5623660.2928322276642865Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC5602170.29171321183251403Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCT5309680.27648282836880406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC5218740.27174744913467713No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA5187640.27012802650237727Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCC5077760.264406413678033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAA5052060.26306817696902646Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT4996550.2601776898205067TruSeq Adapter, Index 1 (100% over 35bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAGAG4968030.25869261157378026No Hit
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCC4897110.25499970311452935Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT4735450.24658182971460677TruSeq Adapter, Index 1 (100% over 35bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA4225300.220017570683489Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCC4208600.21914797717997106Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAAC4202350.2188225305094928Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT4189830.21817059573919073Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCA4131830.21515045063715244Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACAC4124890.2147890746542534No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC4095180.21324203136146794TruSeq Adapter, Index 1 (100% over 38bp)
GGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGT3955750.20598170667910243No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAA3940900.2052084453900461Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCA3933900.20484394511911044Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA3863000.201152078089205No Hit
TATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCCAGT3741580.1948295605324897Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT3490630.18176222581944645Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA3471240.1807525600689547Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA3457480.18003605667922976TruSeq Adapter, Index 1 (100% over 37bp)
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3413790.1777610542739185Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCC3403970.17724971246526305Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3350380.17445920253391423Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGTC3322420.17300328430886266No Hit
TGTCTGAGCGTCGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3259320.16971757472371413Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3251440.16930725156157514TruSeq Adapter, Index 1 (100% over 49bp)
GCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAG3205210.16689998762938152Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG3147370.16388817396210748TruSeq Adapter, Index 1 (100% over 39bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3144030.1637142552614039Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG3082020.16048530357558674Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTG3073800.16005727611457374Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC2961630.15421641963016625Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCTCGGGCCGATCGCACGCCCCAGATCGGAAGAGCACACGTCTGAACTC2948840.15355042556369952Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACGTAGCAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2925550.15233768109082932Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2895530.15077449564318815Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2857380.14878796916658882Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA2815100.14658638753013747Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACGCCTGTCTGAGCGTCGCTGCAGAGCAGCTCAGATCGGAAGAGCACAC2760450.14374068184347552No Hit
TACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACT2746000.1429882491413298Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTC2715070.14137767865846695Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACT2642870.13761811872110205Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGA2607610.13578207878493187Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACG2575840.1341277682695568No Hit
TCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC2570700.13386012092775545Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTCAGATCGGAAGAGCACACGTCTGAACTCC2511150.13075926505143856Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2466140.12841552830932232No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT2414690.12573645131794525Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2387250.12430761025587747No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC2387070.1242982373917677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTG2385430.1242128401854342Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACG2365100.1231542272557025No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGT2326140.12112552289060921No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGAGCA2304080.11997682632248913No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2282730.11886510049613538TruSeq Adapter, Index 1 (100% over 38bp)
GTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGAGCAC2269480.11817515355472147No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2168550.11291958036250209No Hit
CTACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCC2147410.1118187895442764Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGACCGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2136450.11124808626292572TruSeq Adapter, Index 1 (100% over 38bp)
GTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAAC2113180.11003638321940105Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG2075310.10806443675363915Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAG2048900.10668922930286621Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTC2029640.1056863328431204Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2016660.10501044519787113TruSeq Adapter, Index 1 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1956470.10187626358249727No Hit
GTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1941020.101071759413075TruSeq Adapter, Index 1 (100% over 35bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA1940400.10103947510336356No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1934090.10071090414484869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)