FastQCFastQC Report
Fri 30 Jun 2023
SRR1640941.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640941.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169421763
Total Bases8.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCN60583963.575925484850491Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN34052852.009945440126249No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN31355951.8507628208307572Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCN21259621.2548340675689935Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGN19152141.130441547819332No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAN18688551.1030784752251694Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACN15387400.9082304260993909No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATN11746720.6933418583302076TruSeq Adapter, Index 1 (97% over 48bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN11564150.6825657929200041Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN11207710.66152717346TruSeq Adapter, Index 1 (97% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACN11029660.6510178978600287No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN10894320.6430295498695761Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAN10018400.5913289900070277Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN8763480.5172582226050852Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN8530820.5035256302934352Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN8418140.49687477281180226TruSeq Adapter, Index 1 (97% over 35bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAN7461780.4404262987158267TruSeq Adapter, Index 1 (97% over 47bp)
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCN7305010.43117306009854234Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN6907680.40772093724464425Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGN6734790.3975162270032569Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAN6576270.388159695870949Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTN6536760.3858276460031879Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTN6386040.37693150436641365Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCN6214890.36682949639710694Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTN6196880.36576646885677844No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCN6165850.3639349450046745No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCN6088110.3593463963658553No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCN5680550.3352904549812765No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTN5503000.32481069152845493No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTN5324480.31427367451016314Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCN5204820.3072108274543218Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTN4965670.29309516747266995No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN4897950.28909804226272867Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN4810750.28395112379983906Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGN4556800.2689619042625592No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCN4527150.2672118339365882Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN4498700.26553259276377617Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCN4187120.24714180314603382TruSeq Adapter, Index 1 (97% over 36bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGN4167040.24595659531650607No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACN4131670.24386890602714362No Hit
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN4051630.23914460151143627TruSeq Adapter, Index 1 (97% over 35bp)
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAN3980740.23496036928856656Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN3876230.22879174029135796TruSeq Adapter, Index 1 (97% over 37bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCN3810510.22491266367001506Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAN3637280.21468788516856596No Hit
ACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTN3489160.2059452066969696No Hit
GATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN3443640.2032584208204704Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCN3424150.20210803732457913Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACN3307550.1952258046092933No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTN3088630.18230420610131415Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGN3030980.17890145553496573Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN2999090.17701917078976448TruSeq Adapter, Index 1 (97% over 37bp)
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN2961940.1748264182565495TruSeq Adapter, Index 1 (97% over 37bp)
AAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCN2940510.1735615276297178Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TTCTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAN2929170.17289219213236495TruSeq Adapter, Index 1 (97% over 40bp)
TCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAN2835910.16738758644602228Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAN2821830.16655652438228968Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TGTCTGAGCGTCGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN2792710.1648377369322972Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGN2762000.163025100854369TruSeq Adapter, Index 1 (97% over 39bp)
TACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGN2661690.15710437389321702No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCN2596920.15328137035145833Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TACTTTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACN2594980.1531668632205179TruSeq Adapter, Index 1 (97% over 38bp)
AAATACCACTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN2573760.15191436769548902Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTN2569500.1516629242017745TruSeq Adapter, Index 1 (97% over 46bp)
ATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN2552120.15063708196685452TruSeq Adapter, Index 1 (97% over 35bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN2544160.15016724858423294TruSeq Adapter, Index 1 (97% over 35bp)
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAN2493750.14719183390861065TruSeq Adapter, Index 1 (97% over 40bp)
ATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGN2469640.14576875817305712TruSeq Adapter, Index 1 (97% over 39bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTN2465050.14549783666222385TruSeq Adapter, Index 1 (97% over 46bp)
TCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCN2447690.14447317491318987TruSeq Adapter, Index 1 (97% over 36bp)
GTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN2417850.142711889971302Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGN2350160.13871653549018967Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN2345050.13841492134632077TruSeq Adapter, Index 1 (97% over 35bp)
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN2244470.13247825782570802Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCN2230670.13166372256437917Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ACTACTCTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN2213490.13064968518831904TruSeq Adapter, Index 1 (97% over 37bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAN2199800.12984164259936312TruSeq Adapter, Index 1 (97% over 47bp)
AAATACCACTACTCTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTN2188210.12915755102843546Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACN2152480.1270486129931253TruSeq Adapter, Index 1 (97% over 38bp)
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACN2120230.1251450795019764TruSeq Adapter, Index 1 (97% over 38bp)
CTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCN2109550.124514700038861TruSeq Adapter, Index 1 (97% over 36bp)
TCGTACGTAGCAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCN2108170.12443324651272812Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGN2034980.12011325841297024No Hit
GAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCN1980000.11686810271240065TruSeq Adapter, Index 1 (97% over 42bp)
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTN1975860.116623742134002No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTN1937840.11437963846474669Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTN1885080.11126551669752131Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACN1854730.10947412936554084No Hit
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN1817710.10728904999058474Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGN1758300.10378241666626972Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCN1733520.10231979465353574Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAN1702200.10047115375608504Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)