FastQCFastQC Report
Fri 30 Jun 2023
SRR1640943.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640943.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160891291
Total Bases8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG40265442.502648822676176No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG25687421.5965699473441355No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA25573041.5894607993418364Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC21769451.3530533483008722No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC21062821.3091336311049926No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT15998800.9943857060603735No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT15452350.9604217794485843No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA14318380.889941270966618Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG12808490.7960959179574237TruSeq Adapter, Index 1 (100% over 49bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG11792040.7329197203097836No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT11291560.7018130024203734No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG11098200.6897949498086879Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG10340230.6426842581554025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC9261180.5756172346208596No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC9210240.5724511216707187Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG9154900.5690115321406676Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA8225860.511268195368014Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7473840.46452731863528895Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA7429650.46178074362023735Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT7281770.45258944438453164TruSeq Adapter, Index 1 (100% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG7082080.44017795842038454No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC6949270.43192331647087095Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG6909870.4294744580053124No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT6524070.4054955342486499Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT5855890.3639656294385754Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT5663510.35200848751968805No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5333220.33147971943366406Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT5212620.323983974993401Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT5189390.322540142959012Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT5165690.3210670986535872Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC4839230.30077637949962127No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG4747570.2950793651099487No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC4696410.2918995783308122No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC4519330.2808933890647941TruSeq Adapter, Index 1 (100% over 36bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG4455690.2769379232590035No Hit
TACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCA4402110.27360772436091646Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC4357440.2708313155371474TruSeq Adapter, Index 1 (100% over 36bp)
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTC3895880.2421436223045783No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCA3621490.22508924985877574Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC3602390.22390211288689332Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA3465150.21537212974442477Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA2996790.1862617908883583No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC2960820.18402611984759323No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT2956950.18378558476480866Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACG2794130.17366570823277191No Hit
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGA2667140.16577280121395757Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2633630.16369002844286953Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2418930.15034561441862007TruSeq Adapter, Index 1 (100% over 35bp)
CGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2365270.14701044321908013Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2321130.1442669758924366Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAG2317510.14404197925169238Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCA2227520.13844876165484932Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2179300.1354517069541073No Hit
CGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG2140530.13304200536249036Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT2090590.1299380461805108No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2081790.1293910930206906No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2074990.12896844739719318TruSeq Adapter, Index 1 (100% over 38bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT2058620.12795099021239129TruSeq Adapter, Index 1 (100% over 48bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG2005530.12465124666070333No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT1994410.12396009675874874Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1951230.12127629704954011Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTAGATCGGAAGAGCACACGTC1936920.12038687662715068No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1924130.11959192993236656Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT1870890.11628286331545441TruSeq Adapter, Index 1 (100% over 41bp)
ACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1860310.11562527644830695Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG1835250.11406770301818263Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG1829410.11370472501212014No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT1800640.11191656110211708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGGAAGAGCACACGTCT1796490.11165862296424733Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTC1755340.10910099540440632Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC1721210.10697968729705823TruSeq Adapter, Index 1 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1712960.10646691870972681Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA1711100.10635131270094662No Hit
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1701690.105766445742548TruSeq Adapter, Index 1 (100% over 37bp)
TTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA1696710.1054569199771043Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACGTCTG1689290.10499573901734682Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC1683400.10462965332287626No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG1677090.10423746304577791No Hit
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC1671340.10388007887884995TruSeq Adapter, Index 1 (100% over 38bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC1634340.1015803894568787No Hit
GATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1621420.10077736277223359TruSeq Adapter, Index 1 (100% over 35bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA1618170.10057536302570907Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGA1609410.10003089601661536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)