FastQCFastQC Report
Fri 30 Jun 2023
SRR1640944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180147807
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG2319717612.876746259808758No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT120719376.701129034559939TruSeq Adapter, Index 1 (100% over 35bp)
GCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTG72307624.013794072996959Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA51081892.8355543623131645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCG37960212.1071702527025487No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT36254502.0124863357343004No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGG30385861.6867182846139226No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC26672311.4805792223715495No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA26261761.4577896027343813No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCA26032991.4450905860874563No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC18471591.0253574721561833No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG15259610.8470605473426606No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC14661940.8138839014565411No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG13604180.7551676718440431No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG11055000.613662757493351No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATA9233990.5125785405758506No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT8602280.47751233519040287No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGAT8316880.46166978874186354No Hit
CGACCGCTCTCAGATCGGAAGAGCACACGTCTGAAC8089040.4490223963703316Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA7291480.40474986187314504No Hit
ACGGCGCGACCGCTCTCAGATCGGAAGAGCACACGT6571740.36479711351690225No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC6567020.36453510644178977No Hit
GCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAA6180080.3430560772799194Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGC6049290.3357959278405204No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGA5703970.3166272237774174No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTAG5669940.3147382193778246No Hit
GCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGA5554870.3083506867224867Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCGACCGCTCTCCAGATCGGAAGAGCACACGTCT5527470.3068297134474693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGCGACCGCTCTCAGATCGGAAGAGCACACGTC5301160.2942672513354548No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA5291930.29375489427967333TruSeq Adapter, Index 1 (100% over 36bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA5184420.2877870170243038No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC5177770.2874178757002576No Hit
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC5064330.28112082430179125Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTC5053660.28052853288411106No Hit
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACT4974730.27614713067253716Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAG4738130.263013470932788No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGA4660560.25870756228522945No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC4648300.2580270100096195No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG4487790.24911710415658847No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4456050.24735521759640405No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGAT4387490.243549453810448No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGA4180380.2320527831904165No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGA4007840.2224750923556899No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGAT3971180.2204400967256848No Hit
CGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCT3877700.21525102439909244Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAGA3761440.20879743487524108No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATC3724830.20676521474391305No Hit
CGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGA3665000.20344405302696802Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGC3614570.20064468506130634No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA3603410.20002519375659122Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGA3447050.19134565429375444No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGG3442130.1910725452239338No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCA3414960.18956433924283075No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCAGA3284270.18230974080078588No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT3084790.17123661127887058No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGA3006950.16691571493845606No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGA2989410.16594207000255073No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGCAGATCGG2687520.1491841640903239No Hit
GCGCGACCGCTCGAGATCGGAAGAGCACACGTCTGA2622280.14556269341652323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGG2552320.1416792156676101No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCG2530770.14048297573780624No Hit
CGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAA2447460.13585843984212365Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCG2382440.13224918136250197No Hit
GGCGCGACCGCTCTCAGATCGGAAGAGCACACGTCT2365450.13130606691204408Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2357730.13087752991630922No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT2325730.12910121076300418No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAG2321310.12885585667995392No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCG2236050.1241230763358668No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCG2157780.11977831070682976No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGAT2144960.11906667284603692No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT2136890.11861870735956281No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGG2102760.1167241519626159No Hit
AGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAGA2097620.11643883069861627No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCGAGA2076020.11523981527013537No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATC2030250.11269912378117375No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATC1939300.10765049168763959No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAA1935070.10741568449956207No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT1835960.10191409102193513No Hit
TCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCA1832550.10172480201216104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)