FastQCFastQC Report
Sun 2 Jul 2023
SRR1640946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181748943
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT56788593.124562325515148No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG56441533.1054667536635963TruSeq Adapter, Index 1 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG51205442.8173720933276627No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC50087182.7558443627372293No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC40447472.2254583345774943No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC24664001.3570367779250303No Hit
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGGAAGAGCACACGTCT14444120.7947292436248171Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG13273620.7303272184642086No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGAGCACACGTCT13226490.7277340809624406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACGTCTG11842570.6515894840719927Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGGAAGAGCACACGTCT9465340.5207920246336728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGA9445300.5196894047411323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGT9235090.5081234502695292No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCGGAAGAGCACACGTC9036660.49720564262098627No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT7091770.39019594188231405No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7049090.387847647620157Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT6745360.37113613365003173Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTC6611400.363765526823449No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGT6153650.3385796857151461No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG6124240.33696151949560443No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGTCTG6033850.3319881755791009Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC5951760.32747150557018645No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA5844530.3215716088098515Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5690150.3130774741286941No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT5647320.3107209267236289No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGGTAGATCGGAAGAGCACACGTCTG5558080.30581085690275517Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA5547980.3052551452802672No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT5508750.3030966733049997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGTCT4961120.2729655489660812Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT4881130.2685644229578821Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA4817290.26505188533613644Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG4503260.24777365555297892Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT4245160.23357274765553934TruSeq Adapter, Index 1 (100% over 35bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC4223710.23239254821966143No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC4203160.231261867641233No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC3948090.21722767323053976No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT3896810.21440619877497719No Hit
TGATCCTTCGATGTCGGCTCTTCCTATCAGATCGGAAGAGCACACGTCTG3866230.2127236580407513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATACCGTCGTAGTTCCGACCATAAACAGATCGGAAGAGCACACGTCTG3706110.20391370309097204Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGAAGAGCACACGTCTG3700360.20359733261282295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG3700310.2035945815651869No Hit
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGA3689820.2030174117711375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCGGCATTGGCAATTTTTGACAGTCTCTAGATCGGAAGAGCACACGTC3683730.20268233416906306No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTAGATCGGAAGAGCACACGTC3679030.20242373569127167No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG3670520.201955507383611No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA3634160.1999549455426544Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT3516220.19346577437867135No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC3507440.1929826904137759TruSeq Adapter, Index 1 (100% over 36bp)
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG3479930.19146906400440525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC3235840.17803899965459496No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCTG3228280.17762304125201983Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC3221680.1772599029640574No Hit
ATTGATCATCGACACTTCGAACGCACTTAGATCGGAAGAGCACACGTCTG3122960.1718282345113831Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG3039850.16725544313069265No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCAGATCGGAAGAGCACACGTCTGA3032230.16683618347095422Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2936490.16156847745739022No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2916760.16048291406019347No Hit
AAATCTCGCGCCGGGCCGTACCCATATCCGCAGATCGGAAGAGCACACGT2903370.15974618350325154No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTCT2886340.15880917667840302Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCTAATACATGCCGACGGGCGCTGACCAGATCGGAAGAGCACACGTC2863890.15757395628980359No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC2787000.15334339523504134No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2741330.1508305883242468Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2702570.14869797619675842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCTAATACATGCCGACGGGCGCTGACAGATCGGAAGAGCACACGTCT2637380.14511116028883864Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGATCGGAAGAGCACA2605820.14337469902094563No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG2584410.14219670042317661Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGGAAGAGCACACGTCT2576420.14175708301093118Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT2553410.14049105088880764No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA2552710.14045253622190254No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCT2533280.13938347911052226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCAGATCGGAAGAGCACA2529360.13916779697585369No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC2529120.1391545919472005Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG2526680.13902034082255982No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA2455500.13510394940783782Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGACCCGGGGGAGATCGGAAGAG2439440.13422031290712927No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGAGATCGGAAGAGCACACGTCTG2328300.12810528422165293Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGAAGATCGGAAGAGCACACGTCT2305350.1268425533566927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACG2288280.12590334569373535No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACAC2283870.1256607032922332No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG2279400.1254147596335677No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGAGATCGGAAGAGCACACGTC2222370.12227691469985605No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT2212760.12174816334420167No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTAGATCGGAAGAGCACACG2192390.12062738653726311No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT2149340.11825873452259912No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG2123940.116861202323471Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGT2077140.1142862217361011No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2073390.11407989316339517No Hit
ATTGATCATCGACACTTCGAACGCACTTGAGATCGGAAGAGCACACGTCT2004470.11028784910182393Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1962790.10799457579238853TruSeq Adapter, Index 1 (100% over 37bp)
TTGATCCTTCGATGTCGGCTCTTCCTATCAGATCGGAAGAGCACACGTCT1957270.1076908601333654Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTTGCAGATCGGAAGAGCACACGTC1947800.10716981171109204No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA1918950.10558245722507448Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)