FastQCFastQC Report
Sun 2 Jul 2023
SRR1640972.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640972.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152967358
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG66769934.36497896498938No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC65691234.294460652186985No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT47307853.092676151208678No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC39602322.58893926899097No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA35905852.347288367234531No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC24396561.594886668566244No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT16240791.061716055787536No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG15295830.9999407847522606No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG11451920.7486512253156651Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC10036220.6561020685210501No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA9740390.6367626484076426Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT9518860.622280473720413No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG9196190.6011864308985451No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG8882410.5806735578187864No Hit
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACGTCTG8378210.5477122772820591Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC7922980.5179523333337561Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGAGCACACGTCT7073640.4624280691309319Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT6606550.431892796370321No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGATCGGAAGAGCACA6471690.4230765363679747No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG6334700.4141210309718496No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA5725010.3742635079047387Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA5538130.3620465223698248Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGGAAGAGCACACGTCT5384630.3520117017383539Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG5236510.34232859012966677Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG5226650.3416840081659775No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG5163160.33753344945658276Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT4952120.3237370419903572Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG4615840.30175326686364023Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC4156190.2717043723798903No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4005900.2618794004404521Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGT3998970.26142636260998897No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3972370.2596874295233627TruSeq Adapter, Index 1 (100% over 49bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC3946800.25801583106377507No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3882560.2538162422861484Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT3738090.24437174367619008Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCCGCCGGTGAAATACCACTACTCTGATAGATCGGAAGAGCACACGT3664250.23954457002519453No Hit
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTC3515680.22983204037556826No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGTCT3507250.22928094240864122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT3442760.22506501027493722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG3388010.2214858152940054No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACAC3346850.21879504514943637No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC3343310.21856362322738165No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCAGATCGGAAGAGCACA3291120.21515178421268152No Hit
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGAAGAGCACACGTCTG3210940.2099101430515653Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACAC3012630.19694593927679654No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2973550.1943911458547908Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2792060.18252652307690378Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC2783110.18194143092933593No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2696040.17624936687472892TruSeq Adapter, Index 1 (100% over 35bp)
GAAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCT2642670.1727603872193439Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2618590.17118619516197697Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG2523400.16496329890197883No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTC2520400.16476717862905105Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACG2505660.1638035743547326No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA2425420.15855801078815784Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2391470.15633858303285855Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACG2357880.15414268971031062No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCAC2260370.14776812710591497No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGGAAGAGCACACGTCT2193240.14337960913203457Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCT2187890.14302986131198003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC2185450.14287035015666544Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2122270.13874005720880658No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA2111300.13802291074413406Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG2111270.13802094954140479Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2083680.13621729676471236No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC2015290.1317464082762023No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1968440.1286836633473136No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGAGATCGGAAGAGCACACG1951110.12755074190403418No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC1941610.12692969437309626No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACAC1929570.1261425983444128No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGT1863960.12185344797548245Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1827790.11948889121821663TruSeq Adapter, Index 1 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC1826060.11937579519416162No Hit
AAGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGT1789570.11699031894111683No Hit
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTC1712430.11194741298990077Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA1679440.10979074372193837Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG1670560.10921022771407218Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTC1661780.10863624904863689No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT1634180.10683194253770141No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1594880.10426276696234762TruSeq Adapter, Index 1 (100% over 35bp)
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC1558540.10188709672294921Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAA1544890.10099474948112787Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)