FastQCFastQC Report
Mon 10 Jul 2023
SRR1640976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121684635
Total Bases12.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA52964974.352642385786833Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC37281813.063805878203111No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT25859992.125164775322702No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG21439541.7618937674423727No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCA15422051.2673785807057727Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG14315101.1764098236396074Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA13288061.0920080419356148Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC12586801.0343787446952526Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCA11781500.9681994772799376Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC11764180.9667761258436612No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG11749840.9655976697468829Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC11707330.9621042130750527Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA9027250.7418561924436885Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG8901860.7315516868666286Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC7768390.6384035256382206Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT6773390.5566347797320509Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC6697820.5504244640253882No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT5503440.45227074067321643Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC5415470.4450413973793816Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT5278510.43378607331977453TruSeq Adapter, Index 1 (100% over 35bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG5226250.42949136511770775No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGTC4757010.3909293889076464No Hit
TCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG4504750.3701987518802189Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC3942100.3239603751122728Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3581260.29430667232555696Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAG3402600.2796244571058622Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA3225720.2650885216527132Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCACACGTCTGAACTCCAG3187500.2619476156541867Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3171190.2606072656584786Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGT3150970.2589455932542346No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2955170.24285481893420646No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2953050.2426805980886576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCACACGTCTGAACTCC2829990.23256757108241316Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA2828350.2324327964660452Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2762150.2269925040248508TruSeq Adapter, Index 1 (100% over 38bp)
TGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2760000.2268158177899782Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACG2650870.21784755322642008No Hit
ACTACTCTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2639820.2169394681588189TruSeq Adapter, Index 1 (100% over 38bp)
ACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG2550740.20961890546000325Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2525940.2075808502856585Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2507360.20605395249778247TruSeq Adapter, Index 1 (100% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2427850.19951984899326033No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG2399510.1971908778787067No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT2329920.19147199644392243TruSeq Adapter, Index 1 (100% over 48bp)
GGGCCGCCGGTGAAATACCACTACTCTGATAGATCGGAAGAGCACACGTC2313880.1901538349521285No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGATAGATCGGAAGAGCACACGT2287030.18794731150732383No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG2252160.18508170731662218Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT2245200.18450973699349962Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA2235700.183729030374295Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC2191840.18012463118289337No Hit
GCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2137970.17569761375378248Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2133460.17532698355877058TruSeq Adapter, Index 1 (100% over 49bp)
TTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCA2064100.1696270034421355Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT2055860.16894984317453063Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA2047440.16825789057098292No Hit
AAATACCACTACTCTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2026930.16657238606994218Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCC1876020.15417065597476623Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1850870.15210383792497714TruSeq Adapter, Index 1 (100% over 35bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC1834950.1507955379904784No Hit
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG1789750.1470810180759469TruSeq Adapter, Index 1 (100% over 39bp)
TGTCTGAGCGTCGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1744180.14333609169308845Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1698560.13958705632802368Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1680890.13813494201630305No Hit
GCCTGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1632200.13413361514376898Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1632090.13412457538291503Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1567410.12880919600079338No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1525900.12539791897308974TruSeq Adapter, Index 1 (100% over 36bp)
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCA1518330.12477581906704983Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1499010.12318810834251999Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT1408540.11575331593836806No Hit
TAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1320790.10854205216624105Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1314840.10805308328368655TruSeq Adapter, Index 1 (100% over 35bp)
CGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG1302700.10705542240398716Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCTGATCGAGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1297900.1066609601121785Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGA1275830.10484725536629995Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1257030.10330227805671603No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1221410.10037503913291929TruSeq Adapter, Index 1 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)