FastQCFastQC Report
Mon 3 Jul 2023
SRR1640977.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640977.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121270426
Total Bases12.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG78859136.502750307812064No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG42438573.4994987153751733Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA20391621.681499824202811Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT16402501.3525556511197543No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG16207251.3364552706362225No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC15904391.3114813334621254No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG14799241.2203502938135964No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG14185881.169772422503076TruSeq Adapter, Index 1 (100% over 49bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT11409820.9408575838597284No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT10438470.8607597370854456No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT9952590.8206939093295509No Hit
CGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAA9866440.8135899514363049Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT9863700.8133640101173555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCA8734180.7202234120955425Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA8672990.7151776641734564Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTC8571510.706809589338789Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG7841610.646621790542733No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC7507870.6191014782120086No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG7028050.5795353600885347Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAAC6333420.5222559373214374Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG6304200.5198464463215459No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCA6115590.5042936024649571Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5552850.4578898733315244Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAG5200890.42886713369012164Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA4976260.410344068553037Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC4824370.3978191682117122No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC4337560.35767665234391116No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC3893350.3210469467634261No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT3862070.31846758747264564TruSeq Adapter, Index 1 (100% over 48bp)
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT3704490.30547348782299155Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC3623490.2987942006569681No Hit
GGGATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACG3597460.29664775812694844No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC3572300.29457305608871204Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGT3407300.280967100750516No Hit
GCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACT3356170.27675090380238293Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC3318180.27361823566118254No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGGAAGAGCACACGTCT3306400.2726468529103708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCC3278280.2703280682794006Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA3017710.24884137868865078Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAG2998770.24727957993649663Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2960970.24416257925901902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG2898200.23898654400702773No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGATCGGAAGAGCACACG2796390.23059125726168392No Hit
CGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGA2772650.22863364889969132Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAA2666380.21987058905853932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2470390.203709187926824Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC2403380.19818352085280874Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2366600.1951506297174218TruSeq Adapter, Index 1 (100% over 38bp)
CAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG2322480.19151247972032356TruSeq Adapter, Index 1 (100% over 39bp)
CATTTATCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2319810.19129231062485094TruSeq Adapter, Index 1 (100% over 36bp)
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT2314320.19083960338359823Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC2281210.18810934167906693No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT2273250.1874529574094182Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTGAACTCC2252600.18575015148375912Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGTTCTTATCAGTTTAATATCTGATACAGATCGGAAGAGCACACGTC2245340.1851514894488785No Hit
ATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCC2166320.17863547374691338Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA2159220.17805000536569401Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCGGAAGAGCACACGTC2120360.1748455967327104No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG2079020.17143668646797697No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2060750.16993013614052943No Hit
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA2058160.16971656387188744TruSeq Adapter, Index 1 (100% over 47bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1994160.16443910240737505Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCCA1980620.16332258946628916Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG1907210.1572691762458227Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGT1894610.15623017601999684TruSeq Adapter, Index 1 (100% over 46bp)
TATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1877400.1548110336480553Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT1826600.15062204861059858Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1777450.14656912312652387Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTCTGAACTCC1771990.14611888969533265Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCGAGTGTTACAGCCCCCCAGATCGGAAGAGCACACGTCTGAACTC1769660.14592675711389025Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1760180.14514503313445934Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG1696420.1398873621504389No Hit
ATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTC1659590.13685034799828277Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC1653750.13636877963964603No Hit
CGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1642280.13542295959280296Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCT1619930.13357997109699277Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1607060.1325187065806135No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC1598230.13179058181918155Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCA1595510.13156628970693976Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC1583300.13055944901191324TruSeq Adapter, Index 1 (100% over 50bp)
TGGAGGTGATGAACTGTCTGAGCCTGACCAGATCGGAAGAGCACACGTCT1532250.12634984889061082Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1530520.12620719251039822No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA1530350.12619317425338308No Hit
TATCTGTTCTTATCAGTTTAATATCTGATACAGATCGGAAGAGCACACGT1498170.12353960066075796No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCCAGATCGGAAGAGCACAC1464140.12073347544767428No Hit
TATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCCAGT1457690.12020160628445388Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGA1412930.11651068167271054Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGTAGCTCAGTGGTAGAGCATGCTTAGATCGGAAGAGCACACGTC1362580.11235880378617619No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1357670.11195392353944564TruSeq Adapter, Index 1 (100% over 35bp)
TTTATCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC1336420.11020164141255676TruSeq Adapter, Index 1 (100% over 38bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG1304940.10760579005469972No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG1272160.1049027402608448Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1248120.10292039379823734No Hit
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1247270.10285030251316178TruSeq Adapter, Index 1 (100% over 37bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1226570.10114337357073355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)