FastQCFastQC Report
Fri 30 Jun 2023
SRR1640979.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640979.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138075895
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC70878345.133288471532269No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG64595124.678232938486475No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC37763412.7349748484338994No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT34533022.5010172847331535No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT22455351.6263048666097728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA20397961.4773005816837181No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAC20077891.4541198519843017No Hit
TACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCAC17133881.2409030555260931No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG13203510.9562501840020663No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCG11254550.8150988266271966No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT9447450.6842215290366215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA9152270.6628434311434301No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG9051850.6555706193322158No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC8684630.6289751009761696No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAAC8440100.6112652755211183Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG8350350.604765227123822No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG7731620.5599543642284557Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA7469000.5409343897426846No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT7404420.5362572518541343TruSeq Adapter, Index 1 (100% over 35bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG7037630.5096928757912451No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATC6437130.46620230127785883No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCT6063410.4391360273275795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGAT5887810.42641838388952685No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACA5750110.4164456076855413No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCA5702230.4129779495544823No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG5659580.409889068616937No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA5308220.38444219391081985No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG5173240.37466641081703655Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCT5125210.37118788909534134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCACA4833650.3500719658561692No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAC4472040.323882745789915No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC4003770.28996878854198266No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3865910.2799844245079853Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACAC3606280.2611809975955615No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCA3571210.25864109010482966No Hit
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACT3186020.23074411359057279Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC3168960.22950856121555469No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA2997850.21711610125721073No Hit
TCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACA2909470.21071527365439133No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAC2805990.20322084459419945No Hit
AAATACCACTACTCTGATAGATCGGAAGAGCACACG2608400.18891059876888722No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACG2551540.1847925736784107No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACAC2550610.18472521941646655No Hit
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA2534410.18355195162776242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCCGCCGGTGAAATACCACTACTCTGATAGATC2527960.18308481723040795No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCT2433140.1762175794696098Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA2416170.1749885452489734Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAA2392300.17325978585907412No Hit
TGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAAC2385450.17276368188668992Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCACA2356650.1706778724845492No Hit
TTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCA2344240.1697790914192517No Hit
CGTAGACGACCTGCTTAGATCGGAAGAGCACACGTC2320000.16802353517244992No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCA2156200.15616049419777434No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATC2109640.15278843566431344No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2066080.14963364894357556Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAC2061390.14929398067635194No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAC2039230.14768906621970473No Hit
TGAGCGTCGCGAGATCGGAAGAGCACACGTCTGAAC2030980.14709156873471652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCGAGATCG2024510.14662298585861058No Hit
ACTACTCTGATAGATCGGAAGAGCACACGTCTGAAC2000530.14488625983557812Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATACCACTACTCTGAGATCGGAAGAGCACACGTC1994380.14444085261949596No Hit
CTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1946590.14097971264281864No Hit
CATTTATCAGATAGATCGGAAGAGCACACGTCTGAA1942150.1406581503599886Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAA1930520.13981585996599913Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCA1929930.13977312984283027No Hit
GGGCCGCCGGTGAAATACCACTACTCTGATAGATCG1813090.13131111697664533No Hit
CTTCTGATCGAGGCTAGATCGGAAGAGCACACGTCT1813040.13130749577976664Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGAAATAGGAGATGGAATAGATCGGAAGAGCAC1771290.1282837963860383No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCAGATCGGAA1770340.12821499364534264No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT1724720.12491101361320163No Hit
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTG1689640.12237038188309408Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACA1688680.12230085490302271No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGG1684780.12201840154648283No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGG1662900.12043376579235643No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGG1651620.11961682377651797No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGG1585920.11485857107788437No Hit
GCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACG1573550.11396268697008989No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1535570.11121202582101677No Hit
TACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCT1521650.11020388460998207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCTACCATCTGTGGGATTATGACTGAACAGATCG1509730.10934059127409602No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGG1474760.10680792617712165No Hit
TTCTGATCGAGGCTAGATCGGAAGAGCACACGTCTG1464780.10608513528012982Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTCTGAGCGTCGCTCAGATCGGAAGAGCACACGTC1459280.10568680362347099No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC1452350.10518490573608087No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACA1442050.10443893917906526No Hit
GTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTG1410090.10212427013418961Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCG1388540.10056353427946275No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGG1385420.10033757159423085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)