FastQCFastQC Report
Fri 30 Jun 2023
SRR1640980.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640980.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164922470
Total Bases8.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG70469364.2728780377834505TruSeq Adapter, Index 1 (100% over 49bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC50829853.082045157339688No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA34234102.075769299356237Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC17175001.0413984219372898No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA13556290.8219795640945712Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC13377590.8111441697423039No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG13231020.8022569635295906No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCA11540240.6997372765518246Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT11509180.6978539673823707No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG10263130.6223002844912522Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8072900.4894966707689983Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG7291690.4421283527951043No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGAGCACACGTCT7225820.4381343548880878Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA7016980.4254714351537422Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG6941310.4208832186420686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA6917500.41943950997095786Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC6891910.4178878717981849Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC6540690.3965918046218929No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGT5908180.35823984445539775Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5827740.35336240113309No Hit
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGGAAGAGCACACGTCT5795950.3514348287410442Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT4994010.3028095565146459TruSeq Adapter, Index 1 (100% over 35bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT4989050.3025088091392277TruSeq Adapter, Index 1 (100% over 48bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGGAAGAGCACACGTCT4919920.2983171425943354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT4576130.27747159013565587Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4462270.27056774010236445Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA4398040.26667318285980074Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG4378960.26551627561726426No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG4242880.2572651258497402Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG4201830.2547760775108449No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC4055250.24588826495261684Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCGGAAGAGCACACGTC3821530.23171675757706028No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC3568090.21634953684601016Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT3534600.2143188857164218No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG3515240.21314500079946658No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAG3414520.2070378887728276Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGT3328940.20184878385583238No Hit
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGAAGAGCACACGTCTG3254770.19735151917140217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACGTCTG3218070.1951262311315129Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3171200.1922842897029131Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATCGGTCGTCCGACCTGGGTATAGGGAGATCGGAAGAGCACACGTCTG3109320.1885322236563641Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTC3088480.18726859960319536No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT2948530.17878279412138323No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2901280.17591781156321512No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2881860.17474028857317017TruSeq Adapter, Index 1 (100% over 38bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA2839960.17219970086550365Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGTCT2800790.1698246454834202Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA2756710.1671518744534932Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTC2640740.16012008551654605No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGGAAGAGCACACGTCT2621670.15896378461952454Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2514270.15245163378889487No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2482150.1505040519948555No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC2315980.14042840857282818No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGT2270270.13765680322396337No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC2256380.1368145893036892No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT2248240.13632102405451485Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTC2235350.13553944468573628No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT2160450.13099791677871425Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2146410.13014660767571576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC2137230.12958998249298595No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2062630.12506664495141262TruSeq Adapter, Index 1 (100% over 36bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT2024380.12274737335670513No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT1959930.11883947651281236Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA1910370.11583442814068938No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACACG1889050.11454169950280274No Hit
TTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA1849680.11215451721042013Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT1848960.11211086033334329No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1838190.11145782621373546TruSeq Adapter, Index 1 (100% over 35bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG1831030.11102368282502682Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCT1773050.1075080915293107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC1744330.10576666721035648Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC1736470.1052900796356009Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCC1711630.10378391737644968Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1691250.10254818521696892Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG1670890.10131366574851809Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGGAAGAGCACACGTCT1665310.10097532495117251Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAGATCGGAAGAGCAC1664200.10090802059901237No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT1662380.10079766571529034Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)