FastQCFastQC Report
Fri 30 Jun 2023
SRR1640983.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640983.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155542963
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC93160255.989358065655468No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG31274312.010654123902732No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA28703291.8453608859180597No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC25480631.6381731136239186No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT22252321.4306220976387083No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC21806321.401948347865792No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG16153021.0384924967643827No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT14702920.9452642354511404No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA14551530.9355312332580421Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG10683490.686851387805953No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC8236630.529540510296181Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG7646500.4916005104004609No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA7461380.4796989755171373Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC7383420.4746868554895666No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA7272550.46755892132516463Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG6997000.44984355865716663Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT6768820.4351736568114624No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGAGCACACGTCT6071060.39031402532816606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCA5991340.38518875328355423No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGATCGGAAGAGCACA5971780.3839312229123474No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG5923390.38082018535290474No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG5840840.37551297000816425Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG5823040.3743685916539985Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAGATCGGAAGAGCAC5805210.3732222845722696No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGACCCGGGGGAGATCGGAAGAG5333000.3428634698183035No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG5022520.32290242535755215No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG4811530.3093376844055619TruSeq Adapter, Index 1 (100% over 49bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA4735720.3044637898533539Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT4726730.30388581449358143No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA4409140.28346766160035153No Hit
AGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGT4244590.27288859091619594No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4208010.27053682910746657Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACTCTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCACAC4079830.2622960191390979No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT4002940.2573526903946146No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA3522840.22648662029152677Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC3510940.22572155835812382No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC3493570.22460482509903068Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG3477280.2235575260322127Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3444680.22146164208020133Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC3407900.21909702208771734No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT3275270.2105701175308072Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT3261120.20966040103016426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3190820.20514074944039737Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGGAAGAGCACACGTCT2965160.19063286071000204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGGAAGAGCACACGTCT2905180.1867766914019762Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC2897540.18628550878254774No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTC2879950.18515463152132444Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2832820.18212460051953622No Hit
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACGTCTG2788680.17928679936488032Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2777530.17856995562055739Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGGAAGAGCACACGTCT2771410.17817649519766446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGT2768100.17796369225652464Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA2729540.17548463442862408Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGAAGAGCACACGTCTG2601390.1672457531878186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2552110.16407749671066765TruSeq Adapter, Index 1 (100% over 35bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC2492970.16027533177441142No Hit
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTC2448640.15742531534518858No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA2370340.15239133640523486No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2351510.1511807384047326No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC2321090.14922500865564714Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAA2317840.1490160631696337Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACG2295900.1476055204117463No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGAGATCGGAAG2258090.14517468077292575No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC2223430.14294635752824125No Hit
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2137880.13744626942718072Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2094830.13467854537398777No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG2093850.13461554027358988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGAGAGGTGTAGAATAAGTGGGAGGCCCCAGATCGGAAGAGCACACGTC2090990.13443166824589808No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1987010.12774669851184461No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC1981490.12739181264021568Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACAC1959210.1259594109699453No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG1952820.12554859199898358Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1898420.12205116601771307No Hit
TGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGA1881950.1209922945855159Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGT1875030.12054740142760428No Hit
AAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1831010.1177173151832012Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGTCT1829930.11764788099092596Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTC1815730.11673494994434433Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1792070.11521382680616674TruSeq Adapter, Index 1 (100% over 37bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1758320.11304400829756599TruSeq Adapter, Index 1 (100% over 35bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTAGAGATCGGAAGAGCACACG1757720.11300543374630197No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG1727570.11106706254528531No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT1700030.10929649064226711Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1696410.10906375751630758No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCAC1685910.10838870286918735No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT1678900.10793802352858611No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT1675730.10773422131607457Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTCT1668810.10728932815816297Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1640490.10546860933850154Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1616980.10395712983813997TruSeq Adapter, Index 1 (100% over 36bp)
TTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTC1615360.10385297854972712Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA1589870.1022142030301943Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA1587190.10204190336788171Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG1584790.10188760516282565No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1573050.10113282977642646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)