FastQCFastQC Report
Fri 30 Jun 2023
SRR1640986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159425486
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAN73454444.607446515797355No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCN27373041.7169801822024866No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAN27020951.694895256584007No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATN26883311.6862617561661377No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACN26813151.6818609541513332No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAN24662221.546943378927507No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACAN22906251.4367997598577182No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCN14561590.9133790565957567No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGN13751640.8625747579656116No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACN13599680.8530430322790423No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCN12866020.8070240413129429No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATN12632480.7923751915048263No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCN11975490.7511653437895118No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN10810980.6781211882270819Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAN10509960.6592396400159006No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACN9852880.6180241470300426No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAN9753860.6118130949276203No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGN9725830.6100549067794593No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCN9338830.5857802434423816No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTN8712700.5465060962712073No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAN7612520.47749705464281916No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCN6537750.4100818610645478No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCN6334050.39730473206774475No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCAGATCGGAN6091780.3821082910169081No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCN5929180.3719091689016397No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGN5807830.3642974624521452No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN5753160.3608682742231063Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGAN5382410.3376128958452728No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAN5274850.3308661702935No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACN5166430.32406550104542253No Hit
AAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGN4876380.3058720485882665No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN4786970.30026378592943415No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGAN4697080.2946254151610364No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATN4512380.2830400655011959No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAN4284080.2687198958891679No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCN4166180.2613245914771745No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGN4160030.2609388313233682No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAN4105900.2575435147175904No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACN4039640.2533873410930044No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGAN3895010.24431539132958954No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATN3808410.23888338656217115No Hit
CGTGCATTTATCAGATAGATCGGAAGAGCACACGTN3799320.238313214237277No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGN3765130.23616863868302715No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACN3532400.22157059630980208No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCN3452060.21653125147129862No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCN3450260.21641834606042853No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCN3399220.21321685040997773No Hit
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACAN3209660.20132665614078796No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGN3195310.20042654911523994No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCAN3122570.19586391601152153No Hit
ATTAATGATGAGATATAACCTTGACTGAAGAGATCN3052250.19145307796019514No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATN3016220.18919308798594472No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCN3002590.1883381431247448No Hit
ATTGATCATCGACACTTCGAACGCACTAGATCGGAN2987520.18739287393484877No Hit
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCAN2870990.18008350308557317No Hit
TACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACN2845440.17848087350349995No Hit
CCAAGTCCTTCTGATCGAAGATCGGAAGAGCACACN2828000.1773869455226249No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGN2736190.1716281423159657No Hit
CATTTATCAGATAGATCGGAAGAGCACACGTCTGAN2731810.17135340581618175Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAN2709900.1699790960649792No Hit
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACN2568090.1610840314452609No Hit
CGTGCATTTATCAGATCAGATCGGAAGAGCACACGN2504030.1570658533228495No Hit
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGN2473280.15513705255381816No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGN2429970.15242042291782634No Hit
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCN2429000.15235957944641298No Hit
CTACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCN2415730.15152721566738708No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGN2394460.1501930500622717No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAN2230140.1398860405543942No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCTN2221700.1393566396278698No Hit
CCAAGTCCTTCTGATCAGATCGGAAGAGCACACGTN2141750.13434175762838824No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAAN2134750.13390268103055994Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGN2051120.12865697019107725No Hit
CAAGTCCTTCTGATCAGATCGGAAGAGCACACGTCN2010970.12613855227639073No Hit
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACN2003800.12568881238975807Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCN2001610.12555144413986608No Hit
AGGAACCGCAGGTTCAGACATTTGGTGTAGATCGGN2001350.12553513558051815No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTN1956600.12272818161583024Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CAAGTCCTTCTGATCGAAGATCGGAAGAGCACACGN1921790.12054471642005847No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCN1905670.1195335857404882No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTAGAN1898670.1190945091426599No Hit
TTTATCAGATCAGATCGGAAGAGCACACGTCTGAAN1821920.11428034787361414Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTN1819230.11411161700959156Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGN1811490.11362612374285No Hit
CTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGN1775770.11138557858936056No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGN1764260.11066361121207433No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAN1728680.10843184759054146No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGN1692470.10616056707520402No Hit
TACGCCTGTCTGAGCGTCGCAAGATCGGAAGAGCAN1673760.1049869780544373No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGN1648100.10337744869725535No Hit
TACGCCTGTCTGAGCGTCGCTCAGATCGGAAGAGCN1625290.10194668624061776No Hit
CTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGN1612990.10117516593300521No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCAGN1612980.10117453868072261No Hit
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN1612210.10112624025496149Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)