FastQCFastQC Report
Fri 30 Jun 2023
SRR1640987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134671161
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC58324254.330864126136107No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT37328432.7718206127294023Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA21934581.6287510879927736No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG21702481.6115165146604775No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC20415201.5159296057453606No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA15060961.1183507952381877No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG14193771.0539576472501042No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT13823471.02646104016286No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA13134790.9753231428664969No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC11639850.8643164515378314No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG10884320.8082146109960395No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAC10021940.7441786293057947No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG10004920.742914810098058No Hit
TACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCAC9497620.7052452751929569No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA9265520.6880107018606604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG8211700.609759353006543Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT8020110.5955328476005342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCA7427450.5515249103703799No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG6629100.49224347297340076Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG6510470.48343460854250747No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCT6220300.461888050404496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG5904570.43844353580645234No Hit
TGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCT5850570.43443376863736993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA5690280.4225314430904773No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAAC5569850.4135889197539479Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT5388840.4001480316932888TruSeq Adapter, Index 1 (100% over 35bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT4874020.3619200995824191No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCT4809020.3570935279900052Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAC4777490.3547522694929466No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC4727730.35105734330158483No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC4723230.35072319603749463No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCA4516090.33534202619668513No Hit
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACAC4488950.3333267469194834No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA4358210.3236186550734496Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG4293290.31879802387684175No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC4264740.3166780451235584No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACA3749900.2784486279137372No Hit
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTG3613810.268343272098174Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT3602540.267506418838997No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCG3563410.26460082274036384No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAC3521210.2614672639526736No Hit
ACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCACA3510190.2606489744303905No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATC3325540.2469377983605562No Hit
CTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC3313270.2460266901538036No Hit
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA3138750.23306771670291013Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACA3084390.22903121775270058No Hit
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTC2924530.21716082183326538No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGA2856260.21209143656227927No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA2835490.2105491613011341Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAC2717740.20180564122410735No Hit
TCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACA2688460.19963145635909385No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA2687540.1995631418073243No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2589540.19228615694491563No Hit
ATTGATCATCGACACTTCGAACGCACTAGATCGGAA2560170.1901052891346203No Hit
TTTGGAAATAGGAGATGGAATAGATCGGAAGAGCAC2514010.18667768075453064No Hit
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGC2508970.18630343581874964No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAC2469800.18339486952221343No Hit
TCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCACA2457730.18249861230497597No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCA2391460.17757773692914106No Hit
CGTAGACGACCTGCTTAGATCGGAAGAGCACACGTC2378210.17659385887376436No Hit
TTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCA2338980.17368083728037362No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAAC2253370.16732387121842665Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGAT2245930.16677141440846419No Hit
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC2245340.1667276039893946Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACAC2195390.16301856935799344No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGC2161860.1605288009657836No Hit
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACT2139070.15883653071053572Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCA2133160.15839768397036394No Hit
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC1958220.1454075234414887Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCTAAACCATTCGTAGACGACCTGCTTAGATCGG1948960.14471992262693867No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCG1929650.14328605958925386No Hit
TTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCAC1823990.1354402818284161No Hit
ATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTC1785070.1325502792687738Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATCTTTCCCGCCCAGATCGGAAGAGCACACGTCTG1759560.1306560355561203Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA1725110.12809795261214094No Hit
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAAC1662350.12343771210229634Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG1661640.12338499108951767No Hit
TACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACA1654380.12284590017011883No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGG1644460.1221092910901689No Hit
TACTTTGAAAAGATCGGAAGAGCACACGTCTGAACT1638570.12167192944894861Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1635660.12145584755150361No Hit
CTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT1603560.1190722637343269No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAG1595410.11846708591158578No Hit
AAATACCACTACTCTGAGATCGGAAGAGCACACGTC1590880.11813071099906831No Hit
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACT1551540.11520952136144427Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGA1544600.11469419202526962Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACAC1543580.11461845197874251No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGG1534310.1139301086147167No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGG1516130.11258015366779232No Hit
GCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1497090.11116633946595292No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA1492230.11080546042073552No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT1491480.11074976921005382No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCAGATCGGAA1460080.10841816385618003No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACG1448390.10755012351902128No Hit
AAATACCACTACTCTGATAGATCGGAAGAGCACACG1426570.10592988056292171No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCG1424590.10578285576672201No Hit
TGGATACCGCAGCTAGGAATAATGGAATAGATCGGA1418850.10535663236763809No Hit
TTCTGATCGAGGCTAGATCGGAAGAGCACACGTCTG1418750.10534920687288052Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGA1409880.10469056548788495No Hit
GTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCT1402950.10417597870118606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC1396790.10371856822412037No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGG1394260.10353070320675412No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAG1390550.10325521735124865No Hit
CTTCTGATCGAGGCTAGATCGGAAGAGCACACGTCT1388030.10306809488335814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAA1379980.10247034255537457No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGG1367110.1015146813800766No Hit
GCGCGACCGCTCGAGATCGGAAGAGCACACGTCTGA1364800.10134315245117699Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA1353640.1005144672362333Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)