FastQCFastQC Report
Fri 30 Jun 2023
SRR1640989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225228521
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT119136305.289574316389531No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG97277954.319077777898298No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC68736983.0518772531477043No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT33481371.4865510749413482TruSeq Adapter, Index 1 (100% over 35bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG31557901.4011502566320186No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG31334061.3912119060622876No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT22989571.0207219715304174No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC17839000.7920400098884457No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA16858830.7485210987111175No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG16063750.7132200632796413No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC14530380.6451394315198651No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT14232940.6319332887685215No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGG13950500.6193931362715825No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACA10459010.464373248714802No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCA10227810.4541081189269098No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGAT9867840.4381256848017041No Hit
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCAC9149070.4062127637911364No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGA8432590.37440151729274107No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATC8109180.36004232341427134No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA8094060.3593710052378313No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGG7946280.35280966925143553No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC7911330.3512579119586724No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCAC7826030.34747064737862393No Hit
GCATTTATCAGATCAAAACCAACAGATCGGAAGAGC7782230.345525955835762No Hit
GGGATGCGTGCATTTATCAGATCAAAACCAACAGAT7627710.33866536822838705No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCG7538850.3347200419612932No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG7196740.31953058023233216No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA6851840.3042172443160518TruSeq Adapter, Index 1 (100% over 36bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGAT6669240.29610992295243105No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAC6372530.2829361917268018No Hit
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCA6045970.26843713989490703No Hit
CGTGCATTTATCAGATAGATCGGAAGAGCACACGTC5902600.26207160504330623No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCG5846700.25958968136189114No Hit
CGTGCATTTATCAGATCAGATCGGAAGAGCACACGT5779280.25659627716509315No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATC5630910.2500087455620241No Hit
ATTTATCAGATCAAAACCAACCAGATCGGAAGAGCA5544020.24615088601500873No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACAC4665210.20713229298344504No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC4571170.20295697808183003No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGG4438170.1970518644927744No Hit
CGTGCATTTATCAGATCAAAACCAACAGATCGGAAG4310380.19137807151874872No Hit
TTTATCAGATCAGATCGGAAGAGCACACGTCTGAAC4274050.18976504312258038Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTAATGATGAGATATAACCTTGACTGAAGAGATCG4198330.1864031243183451No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCG4152150.18435276232178427No Hit
CATTTATCAGATCAAAACCAACCAGATCGGAAGAGC4113460.18263495145892292No Hit
GGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCAC4095430.18183443117312836No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA3853190.17107913255799428No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT3830870.17008813905943998No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT3675170.16317516021871847Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGA3673210.16308813749214293No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG3642990.16174638912626876No Hit
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGA3577830.15885332746113445No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT3570980.15854919191162295No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAA3487440.15484007018809132No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3441570.15280347199012154Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT3367360.14950859620482967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCAGA3282980.14576217902705138No Hit
GGGACATGTGGCGTACGGAAGACCCGCAGATCGGAA3225040.1431896806710372No Hit
GCATTTATCAGATCAAAACCAACCAGATCGGAAGAG3202220.1421764874973361No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCG3186150.14146298993811712No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGG3136230.13924657437145804No Hit
TATCAGATCAAAACCAACCAGATCGGAAGAGCACAC3121460.1385907959676208No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATC3119430.1385006652865247No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTAGATCGGAA3033350.1346787692132472No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGG3007530.13353237798866513No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGA2968190.13178570754811286No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA2892890.12844243647100093No Hit
CGGGGGGGATGCGTGCATTTATCAGATAGATCGGAA2846860.12639873437698415No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG2838590.12603155175005568Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACG2837880.12600002821134718No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCAGATCGGAA2724400.12096158994002362No Hit
CATTTATCAGATCAGATCGGAAGAGCACACGTCTGA2716790.12062371088428893Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACT2656920.11796552178220804Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCG2646640.11750909646118929No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCG2589830.11498676937100696No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCA2586990.11486067521617299No Hit
AAATGGATTTTTGGAAATAGGAGATGGAAAGATCGG2573570.1142648359352322No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGG2534510.11253059731276217No Hit
TATCAGATCAAAACCAACAGATCGGAAGAGCACACG2502590.11111337005138883No Hit
GTGCATTTATCAGATCAAAACCAACAGATCGGAAGA2494720.11076394716457778No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCG2411410.10706503729161371No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGA2387860.1060194325921982No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGA2385270.10590443827493766No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAG2384540.1058720267492233No Hit
GGATGCGTGCATTTATCAGATCAAAACCAACAGATC2352580.1044530235138382No Hit
TGCGTGCATTTATCAGATAGATCGGAAGAGCACACG2351030.10438420452088303No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA2347430.1042243668598259Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCAC2294990.10189606493042681No Hit
AAGCTACCATCTGTGGGATTATGACTGAACGAGATC2281820.10131132548705941No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAGATCG2275410.10102672565167711No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGG2265960.10060715179140212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)