FastQCFastQC Report
Fri 30 Jun 2023
SRR1640991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123051889
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT47818683.8860581815204807No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC31888502.5914677343961783No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT26010652.1137952624197425No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT20223811.6435188573171764TruSeq Adapter, Index 1 (100% over 35bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC16152481.312655996690957No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT14524641.1803670888790663No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT14332571.1647582265071934No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG13733471.116071448525264No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA13493911.0966032386548734No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC13181151.0711863188057196No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACAC11493350.9340246698691477No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG10841420.8810445811197584No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA10617830.8628741977297074No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATC9977770.810858742688623No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAC8397680.6824503116729886No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGAT7530590.6119849163794633No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT7499650.6094705299485488Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACG7497600.6093039335625314No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG7353160.5975657960033429No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCA6882750.559337207736811No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGA6724070.5464418347937755No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCA6690310.543698276748925No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT6524030.5301852781796792No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC6030300.4900615544390383No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAC5700970.4632980481916861No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA5680400.46162639567442965Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG5551860.4511803959384972No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG5415670.44011270724986595No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA4842790.3935567376783627Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATC4738650.3850936412686846No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA4450740.36169619468417913TruSeq Adapter, Index 1 (100% over 36bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG3907180.3175229597653718No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCG3715240.3019246620423682No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC3712520.30170361708140864No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCG3635810.29546966158317167No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGA3618370.29405237330407824No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAC3603600.2928520666594562No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACA3566150.28980863511977456No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGA3526180.28656041192508636No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCCAGAT3385400.27511970986483597No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGG3129800.2543479848570224No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCA3088110.25095998323113916No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC3025820.2458978910921067No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA2894850.23525441368884634No Hit
AAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGT2866000.23290987430513968No Hit
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAAC2853730.23191273398492893Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGAT2770150.2251204774272096No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGAT2594680.2108606394494277No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA2511250.2040805728711731No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2414260.1961985321493114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCG2384640.19379141753768608No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA2370880.19267319008812617No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGC2245100.1824514859743437No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA2223920.18073026087393101No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGAT2192220.17815411188039543No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGAT2184310.17751129362995802No Hit
CGTGCATTTATCAGATAGATCGGAAGAGCACACGTC2169160.1762801057040254No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2134660.17347641042714915No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAC2107760.1712903407764833No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAAC2086850.169591057639107Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCAC2057700.167222138296471No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAC2042940.16602264431714656No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACA1989180.1616537556770055No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCT1950680.15852499428107114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGA1939590.1576237484659825No Hit
TTTCGTACGTAGCAGAAGATCGGAAGAGCACACGTC1881760.1529241050496998No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCCAGA1855400.15078191932510682No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGG1767640.14364996867297178No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG1751260.1423188229154288Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCG1714130.13930139666527183No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGG1665270.13533071402097696No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCAC1589020.12913414112643162No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA1552320.12615165948407342Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGAT1526290.12403629171430273No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGC1513240.12297576350087562No Hit
GGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGA1504470.12226305603484072No Hit
TCGTACGTAGCAGAAGATCGGAAGAGCACACGTCTG1500790.12196399520530725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAG1494480.12145120340249307No Hit
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACT1493520.12137318753391914Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGC1450750.11789741805589021No Hit
GATGGAATAGATCGGAAGAGCACACGTCTGAACTCC1420940.11547486280360962Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGAT1416520.11511566474205041No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC1409100.11451266709119759No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAA1398620.11366099385926534No Hit
ATTTATCAGATCAAAACCAACCAGATCGGAAGAGCA1392850.113192085982524No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGG1388930.11287352118584705No Hit
GTTTCGTACGTAGCAGAGCAGATCGGAAGAGCACAC1382350.11233878741999646No Hit
GAGATGGAATAGATCGGAAGAGCACACGTCTGAACT1349130.1096391133012188Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTC1343120.10915070145733399No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1304120.10598130679651736No Hit
TTTATCAGATCAGATCGGAAGAGCACACGTCTGAAC1298170.10549777094441841Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGG1298060.10548883162614432No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAG1295490.10527997664464947No Hit
CCAAGTCCTTCTGATCAGATCGGAAGAGCACACGTC1295320.1052661613345895No Hit
CCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG1282890.10425601836961641No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1272340.10339865648060065Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAG1265510.1028436060823089No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCG1263300.1026640070515293No Hit
TACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACA1253130.10183752644382403No Hit
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1241820.10091840199218721Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGG1240520.10081275550349333No Hit
CAAGTCCTTCTGATCAGATCGGAAGAGCACACGTCT1234180.10029752570478621Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCAC1232600.10016912458775824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)