FastQCFastQC Report
Sun 2 Jul 2023
SRR1640994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224349896
Total Bases8 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG76237693.3981602558888637No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC63398922.8258947800002545No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG45394622.0233849361802245No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA41352981.8432359781437118Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT41098451.8318907533614366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC33268041.482864070505297No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA31518241.404869828867672No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT30936751.3789509400976054No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACA28324261.26250381680587No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA25846491.1520615993510424No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA23702571.0565001554536044Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG20695940.922484938437413No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC20614020.9188334992586759No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA18935240.8440048485692189No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAC17707070.7892613420244242No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC15435020.6879887298900286No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGCC14699920.6552229469275083No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCAC14137730.6301643215381745No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG13942140.6214462430595467No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCT13495980.6015594497980066Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCAC13474830.6006167259377735No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA12331010.5496329715258705No Hit
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC11929090.5317180980551914Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCT11044450.492286833955118Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGA10935260.4874198827353145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTG10920010.48674014094483914Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATCAGATCAAAACCAACAGATCGGAAGAGCACACG10494150.46775818429619415No Hit
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTC10479740.4671158840207352No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGA9492320.42310338311901874No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCA9170560.4087614999384711No Hit
TCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACA9108270.4059850333070803No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCG8860150.3949255229429658No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG8842250.3941276620872603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCT8334290.3714862430780891Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA8247220.367605251753716No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG8236490.3671269809726143No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAAC7798320.34759632783605127Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCGACCGCTCGAGATCGGAAGAGCACACGTCTGA7618770.33959320400130694Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGATACCGCAGCTAGGAATAATGGAATAGATCGGA7382700.3290708010847484No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACA7188360.3204084391463235No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCA7056050.3145109547989271No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGC7010720.3124904501850092No Hit
ACCTCGGGCCGATCGCACAGATCGGAAGAGCACACG6993630.31172869364735517No Hit
CGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAA6778420.30213608835370265Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCACA6624040.2952548727724839No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT6609120.2945898401486221Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG6484060.2890155117344026Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATC6453900.2876711830523871No Hit
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACT6268990.27942914669325275Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT6113330.27249087737486627No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGAT5981970.2666357375980241No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG5861800.2612793722890783No Hit
TTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCA5493740.24487374845941537No Hit
ACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCACA5438950.24243158106924198No Hit
CGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCT5374950.23957889421085354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG5240620.23359137193448934No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCA5143420.22925885376831198No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAC4987920.22232771616707148No Hit
TTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCAC4923620.2194616573390344No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAAC4893370.21811331706612425Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATACCGCAGCTAGGAATAATGGAATAGATCGGAAGA4789350.21347680945659986No Hit
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCAC4785720.21331500862385067No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4697480.2093818666178477No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAC4560400.20327176795303706No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAC4200800.18724323366746737No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAC4152010.18506850567026784No Hit
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC4072010.18150264709728237No Hit
TTTCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCA4039520.18005446278432863No Hit
GCTAGGAATAATGGAATAGATCGGAAGAGCACACGT4028760.1795748548062621No Hit
GCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA4023860.17935644596866673No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAG3996470.17813558513974082No Hit
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTC3968200.17687549986651208No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGAT3947640.17595907421325482No Hit
TCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACAC3929200.17513714381218168No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT3924490.17492720388869715TruSeq Adapter, Index 1 (100% over 35bp)
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA3903670.17399918919507767Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC3843760.17132880685623317No Hit
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCA3806170.1696532990592516No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC3805040.1696029313069082No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGA3759710.1675824266929903No Hit
CTCGGGCCGATCGCACAGATCGGAAGAGCACACGTC3694260.16466510864796657No Hit
CGTGCATTTATCAGATCAAAACCAACAGATCGGAAG3672310.16368672620200367No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAA3454890.1539956140652724No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCCAG3445860.15359311777884666No Hit
ATTTATCAGATCAAAACCAACCAGATCGGAAGAGCA3435060.15311172687149363No Hit
TTTGGAAATAGGAGATGGAATAGATCGGAAGAGCAC3318160.1479011160317186No Hit
TATCAGATCAAAACCAACCAGATCGGAAGAGCACAC3315740.14779324880988579No Hit
GCGACCGCTCCAGATCGGAAGAGCACACGTCTGAAC3231950.14405845768700512Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA3193030.1423236674912477No Hit
CACGGCGCGACCGCTCTAGATCGGAAGAGCACACGT3154660.1406133925731795No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCAGA3063430.1365469766030112No Hit
TACCGCAGCTAGGAATAATGGAATAGATCGGAAGAG3040000.1355026257734481No Hit
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGC2973520.13253939729929715No Hit
GGATACCGCAGCTAGGAATAATGGAATAGATCGGAA2840500.12661026595706557No Hit
TCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGG2807760.12515093833607127No Hit
TCGTACGTAGCAGAGCAAGATCGGAAGAGCACACGT2800290.12481797629181875No Hit
ACCTCGGGCCGATCGCACGCCCCAGATCGGAAGAGC2736350.12196796382736011No Hit
ACGGCGCGACCGCTCCAGATCGGAAGAGCACACGTC2711700.12086923365455896No Hit
TGTCTGAGCGTCGCTCAGATCGGAAGAGCACACGTC2674740.11922180699383965No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA2649610.1181016816696006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC2588480.11537691998751806No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG2564790.11432098011759276No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTAG2537590.11310858820277768No Hit
CGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA2522100.11241814883658338No Hit
GTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTG2421470.10793274448408925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2381900.10616898168742633No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2308610.10290220950225No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC2276960.10149146670431262No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCA2256640.10058573862677431No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)