FastQCFastQC Report
Thu 29 Jun 2023
SRR1640996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1640996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7500448
Total Bases375 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG129524517.268901804265557TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG3549794.732770629167751TruSeq Adapter, Index 1 (97% over 49bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2957833.9435377726770455No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2650653.533988903062857No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2000302.6669073634001594No Hit
CCCCCGCGGGGGCGCGCCGGCAGATCGGAAGAGCACACGTCTGAACTCCA1593202.1241397847168595Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAGAGAACGCGGTCTGAGTGGTAGATCGGAAGAGCACACGTCTGAACTC1004891.339773304207962Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGA580380.7737937787182845Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA561820.7490485901642142Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT389990.5199556079850164No Hit
AGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGAACTC372070.49606370179487946Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACGTCTG369320.4923972541373529Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT367990.49062402672480365TruSeq Adapter, Index 1 (100% over 35bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA364690.4862242895357717Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC332900.44384015461476434TruSeq Adapter, Index 1 (100% over 36bp)
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGGAAGAGCACACGTCT307940.41056214242135936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC294660.3928565333697401Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC284570.3794040036008516No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC262380.3498191041388461Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT248290.3310335595953735Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGAGAACGCGGTCTGAGTGGAGATCGGAAGAGCACACGTCTGAACTCC235500.3139812448536407Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGTCTGAAC231270.30834158172951803Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGCGAGAATTAATGTGAATTGCAGATCGGAAGAGCACACGTCTGAAC211810.2823964648511662Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGAAGAGCACACGTCTG210480.280623237438617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCAGATCGGAAGAGCACA206820.2757435289198725No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG205770.2743436125415442Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTCAGTGATGAGGCCTGGAATGAGATCGGAAGAGCACACGTCTGAACTC194810.2597311520591837Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC179790.23970568158062025TruSeq Adapter, Index 1 (100% over 50bp)
CTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGAAC177650.23685251867621776Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGTCTG175130.23349271936822974Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT173110.23079954690706475TruSeq Adapter, Index 1 (100% over 48bp)
AGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAAC172820.2304129033359074Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGTAGGTAAGGGAAGTCGGCAGATCGGAAGAGCACACGTCTGAACTCC172320.22974627648908438Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA170920.22787972131797993Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATACCGTCGTAGTTCCGACCATAAACAGATCGGAAGAGCACACGTCTG159610.21280062204284328Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA156820.2090808442375709TruSeq Adapter, Index 1 (100% over 37bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGGAAGAGCACACGTCT155570.20741427712051333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCGGCGGCGGCGGCGGCGGCGGGACAGATCGGAAGAGCACACGTCTGA151250.2016546211639625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC147240.19630827385244187No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA146200.19492169001105003Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACT142580.19009531164005136Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCCCGAGTGTTACAGCCCCCCAGATCGGAAGAGCACACGTCTGAACTC141280.18836208183831152Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGGTGTAGAATAAGTGGGAGGCCCCAGATCGGAAGAGCACACGTCTGA139470.1859488926528122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGAACTC138990.1853089308798621Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGAA132810.17706942305312962Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG132770.17701609290538378Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGCAGCAGGTCTCCAAGGTGAACAGATCGGAAGAGCACACGTCTGAA131320.17508287504959702Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCATTGATGATCGTTCTTCTCTCCGAGATCGGAAGAGCACACGTCTGA130080.17342964046947595Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGCCGTC125510.16733667108951358TruSeq Adapter, Index 1 (98% over 50bp)
CGCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTG125270.16701669020303853Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGAGCACACGTCT124770.16635006335621552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCGTTGATGAGGCTCAGAGTGAGATCGGAAGAGCACACGTCTGAACTC124340.1657767642679477Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCCGCGGGGGCGCGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG113020.15068433245587462Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGACATGTGGCGTACGGAAGACCCGCAGATCGGAAGAGCACACGTCTGA109430.1458979516956854Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCCGGGGGAGAGGGTGAGATCGGAAGAGCACACGTCTGAACTCC108420.1445513654651029Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA107330.1430981189390287Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGAAAATCCGGGGGAGAGGGTGAGATCGGAAGAGCACACGTCTGAACTC105020.1400183029067064Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAACT103060.13740512566716015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACAC102970.137285132834732No Hit
ACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGAAC99910.13320537653217515Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCC97950.1305921992926289Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGTCT97100.1294589336530298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCGAACGTCTGCCCTATCAACTTTAGATCGGAAGAGCACACGTCTGAA92580.12343262695774973Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCC91130.12149940910196298Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAACT88840.11844625814351356Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA88040.11737965518859675Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGCTAATACATGCCGACGGGCGCTGACCAGATCGGAAGAGCACACGTC85660.11420651139771917No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC84950.11325990127523049No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG84810.11307324575812006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGAGCATAGGGCTCTGCCCCATGATGTAGATCGGAAGAGCACACGTC83760.11167332937979171No Hit
CAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAAC82340.10978010913481435Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGATCCTTCGATGTCGGCTCTTCCTATCAGATCGGAAGAGCACACGTCTG78570.1047537427097688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTCCCCGCCCCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC76440.10191391234230275Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)