FastQCFastQC Report
Thu 6 Jul 2023
SRR1642044.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1642044.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116792850
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT74731606.398645122539608No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA65431245.602332677043158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG64525175.524753441670445No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT49243044.216271800885071TruSeq Adapter, Index 1 (100% over 35bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC31519752.698773940356794No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCG25415502.17611780173187No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG20947541.793563561468018No Hit
GCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTG19051451.6312171507074278Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG15523511.329149001843863No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC14923821.277802536713506No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG13589351.1635429737351215No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA11688391.0007795853941401No Hit
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACT9893220.8470741145541015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC9868290.8449395660778892Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA8734610.7478719801768687No Hit
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTC8226080.704330787372686No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG7733740.6621758095636847No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC7418470.6351818625883349No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC6714030.5748665265039768No Hit
CGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCT6181700.5292875377217012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGG6140760.5257821861526626No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC6048400.5178741678107864No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA5972040.5113360963449389Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA5547020.47494517001682895No Hit
CGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAA5112390.43773141934630416Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG4650150.3981536540978322No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAG4152870.3555757051908571No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGC4089450.350145578260998No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC3846620.32935406576686843No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3335560.2855962501129136Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACCGCTCTCAGATCGGAAGAGCACACGTCTGAAC3192130.27331553258611296Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3140550.26889916634451505Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT3066690.2625751490780472Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT3029530.25939344745847026No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC2751390.23557863345230468No Hit
GGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTG2745320.23505890985621122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCG2617080.2240787856448404No Hit
GCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAA2541910.21764260397789761Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCA2497080.21380418407462443No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2417960.20702979677266203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGA2386910.20437124361636863No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAGA2363710.20238482064612687No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATC2356050.20172895857922807No Hit
ACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCC2313390.19807633772101632Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT2212600.1894465286188324No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2182090.18683421116960497No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA2115440.18112752621414752Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG2107840.18047680144803385Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCGCGACCGCTCTCAGATCGGAAGAGCACACGT1990280.17041111677641227No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGA1935820.16574816009712923No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA1913690.1638533523242219TruSeq Adapter, Index 3 (100% over 36bp)
CACGGCGCGACCGCTCTAGATCGGAAGAGCACACGT1895870.16232757399104483No Hit
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC1892790.1620638592174093Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG1779410.15235607316715022No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGA1771280.15165996891076808No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGAT1706960.1461527824691323No Hit
GCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGA1704130.14591047311543473Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGA1675010.1434171698010623Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGCGACCGCTCTCAGATCGGAAGAGCACACGTC1615450.1383175425550451No Hit
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGC1601830.13715137527682558No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCG1577030.13502795761898095No Hit
GACCGCTCTAGATCGGAAGAGCACACGTCTGAACTC1503260.12871164630369067Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA1490100.127584864998157No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGAT1445960.12380552405391256No Hit
CGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA1427030.12218470565621097Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA1310930.11224402863702701Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTCGGCATTGGCAATTTTTGACAGTCTCTAGATCG1245150.10661183454295362No Hit
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAA1202280.10294123313199395Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAG1179700.10100789560319831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)