FastQCFastQC Report
Fri 30 Jun 2023
SRR1642046.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1642046.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71186914
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1433695120.13986868429217TruSeq Adapter, Index 1 (100% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA61342928.617162418362453TruSeq Adapter, Index 1 (100% over 36bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT27173223.817165048059254No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC11646191.6360015269098476No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG8977591.2611292575486555No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG5270070.7403144347569274No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATC4871500.6843252117938418No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG2768950.3889689613459013No Hit
ATTGATCATCGACACTTCGAACGCACTAGATCGGAA2588310.3635935110208598No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA2136850.3001745517441591TruSeq Adapter, Index 3 (100% over 36bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAG1732840.24342114338598808No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG1687000.23698175763034202Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT1554150.21831961981102316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGG1541650.2165636791054041No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA1462150.20539589621766718Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT1309680.18397763386680874Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG1243770.17471890971422077No Hit
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGA1082810.15210801243610586No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1069110.15018350142274745No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGA996730.14001590236093112No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGATCGGA981290.1378469644013505No Hit
CCCCCGCGGGGGCGCGCCGGCAGATCGGAAGAGCAC980320.13771070340259448No Hit
AGCTGCGAGAATTAATGTGAATTGCAGATCGGAAGA940260.13208326462922665No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGG824560.11583027745801708No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATC794260.11157387718759659TruSeq Adapter, Index 1 (100% over 36bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAG784680.11022812423081019No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA764970.1074593569261901Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC764180.107348381473595No Hit
AGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGC761970.10703793115684156No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA759630.10670921905674967No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCAGATCG738510.10374238164053579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)