FastQCFastQC Report
Thu 13 Jul 2023
SRR1642049.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1642049.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183726008
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT67392633.6681050621858615TruSeq Adapter, Index 1 (100% over 35bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC48760612.6539851668686993No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT44210252.406314189333499No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG43152392.3487360591865687No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG35298121.9212369758776886No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG22331281.2154664569863185No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT12593590.6854549411425737No Hit
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGG12587310.6851131278049649No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGG11510860.6265231648640621No Hit
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGA10580680.5758945135301693No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGG8173910.44489672904665734No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCG8123940.44217691814215No Hit
ATTGATCATCGACACTTCGAACGCACTAGATCGGAA8071810.4393395408667454No Hit
AGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAGATC7763110.4225373470260128No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC6636790.36123301606814423No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATC5829190.31727625628267064No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG5816140.31656595945849975No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT5624320.30612541257631853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATC5500820.29940344646251715No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGATCGGA5430820.2955934251834395No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC5410520.29448851901250694No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCAGATCG5388740.2933030581059596No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCG5201770.28312649126954303No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC5023000.2733962412115328No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT4958500.2698855787472398No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGGTAGATCGGA4813300.26198250603692425No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGG4637470.2524122768726353No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTAACAT4605680.25068198292317984TruSeq Adapter, Index 1 (97% over 35bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG4584530.24953081220814421No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGAGATCGG4457030.24259113059268123No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC4447130.24205228472606882No Hit
AGATCGGAAGAGCACACGTCTTAACTCCAGTCACAT4412970.2401929943418789TruSeq Adapter, Index 1 (97% over 35bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGG4355900.2370867384219223No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA3966180.21587471709503422No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG3960030.21553997951122955Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA3609300.19645014003678782No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGA3572970.1944727389929465No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCG3564740.1940247893482778No Hit
AGATCGGAATAGCACACGTCTGAACTCCAGTCACAT3528710.1920637169670611TruSeq Adapter, Index 1 (97% over 35bp)
TGATCCTTCGATGTCGGCTCTTCCTATCAGATCGGA3417280.18599870737952354No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGAT3380830.18401477487063236No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT3350830.1823819086081705No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA3341200.18185775853792022Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCAC3338570.18171461059557772No Hit
AGTCGGCATTGGCAATTTTTGACAGTCTCTAGATCG3300770.1796571991048758No Hit
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGA3262020.1775480801825292No Hit
AGATACCGTCGTAGTTCCGACCATAAACAGATCGGA3253250.17707073894513617No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATC3167430.1723996528569869No Hit
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAA3098290.16863644041076645No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGA3087670.16805840575385494No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTAGATCG3066470.16690451359504854No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATC3064020.16677116285028082No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT2948250.16046993194344047No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGA2889880.15729291848544383No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCAGATCGGA2870430.15623427685861438No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCG2837070.15441852957475677No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCG2743160.14930711388449697No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT2717160.14789196312369668Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCTCGCGCCGGGCCGTACCCATATCCGCAGATC2709540.14747721509303136No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATC2650910.1442860501274267No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCAGATCGGAA2649900.14423107696325715No Hit
ATTGATCATCGACACTTCGAACGCACTTAGATCGGA2646870.14406615747074852No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCAGATCG2513870.13682711704050088No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGAGATCGG2496270.13586916883318992No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGAT2319170.12622981499712332No Hit
AGAGCTAATACATGCCGACGGGCGCTGACAGATCGG2304460.12542916623976286No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA2302880.12534316861660652No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGG2282270.12422138949429523No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATC2275380.12384637454268316No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAG2265530.12331025011984151No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGA2256700.12282964314992355No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAG2248000.1223561119338096No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAC2192540.11933748650327176No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGACCCGGG2164780.11782654092174039No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGG2137440.11633845546788345No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCAG2121640.11547847923632021No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGA2120020.11539030445814726No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGAGATCGG2061520.11220621524634662No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGAAGATCGG2012310.109527770287155No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGAT2007950.10929046039034386No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA1990580.10834503082437844No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATC1960370.10670073449807933No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGAGATCGGA1949860.10612868701746353No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATC1943220.10576727928470529No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGAGATCG1927730.10492417600452082No Hit
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAA1909560.10393520333822306Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTTATCGAGGCCAGATC1907240.10380892834725935No Hit
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1905450.1037115006602658TruSeq Adapter, Index 1 (97% over 35bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGA1899780.10340288893666051No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGAT1894950.10313999746840415No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1868700.10171123948875TruSeq Adapter, Index 1 (100% over 36bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCG1844390.10038807352740174No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)