FastQCFastQC Report
Fri 30 Jun 2023
SRR1642050.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1642050.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72320910
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA50631537.000953113007012Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT48148166.657571095275211No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG27089583.745746562093868No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC26883623.7172679381384994No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT19051962.63436397578515TruSeq Adapter, Index 1 (100% over 35bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG16502342.2818213985415836No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG14275911.9739671417298261No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG10498661.4516769769628175No Hit
GCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTG9166971.267540743057575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCG7436141.0282143850236398No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC5661180.7827860573103961No Hit
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC5290580.7315422330830738Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACT5072790.7014278443122466Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTC5052210.6985821942782523No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA4749740.6567588820439345No Hit
CGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCT3657850.505780416756371Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG3374170.46655524660848435No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG3109170.42991300856142434No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC3095770.42806015576961076No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2857660.39513606783985433No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC2755630.38102811482875426No Hit
CGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAA2492830.3446900764937831Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG2439250.33728143077845674No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA2301180.31819013339295643No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGG2256960.31207571917997157No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA2091890.28925106169156334TruSeq Adapter, Index 3 (100% over 36bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGACCCGGG2085520.28837026525246984No Hit
GGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTG1967440.27204303706908556Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAG1893180.2617749140601245No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCG1793810.24803476615545905No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGA1780780.24623307422431492No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA1740640.2406828122046584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT1738760.24042285972341887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG1618590.22380664181355017Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA1473640.2037640289647904No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGC1303470.18023418123472174No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA1263620.17472401826802236No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCA1041640.14403026731826246No Hit
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAA1023070.14146254520304016Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACCGCTCTCAGATCGGAAGAGCACACGTCTGAAC1003280.13872613052020502Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCGGCATTGGCAATTTTTGACAGTCTCTAGATCG959090.13261586448511226No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC919450.12713473876365772Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACGGCGCGACCGCTCTAGATCGGAAGAGCACACGT901500.12465274565820592No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAC895130.12377194921911241No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGA885050.1223781614473601No Hit
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC876310.12116965895478916Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAA870180.12032204793883262Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAGA866120.11976066119743238No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGAT859240.11880934573417286No Hit
ATTGATCATCGACACTTCGAACGCACTAGATCGGAA844100.1167158986246163No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC814080.11256495528056823No Hit
CGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA804700.11126795832629871Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCGCGACCGCTCTCAGATCGGAAGAGCACACGT790580.10931554926507424No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCG778870.1076963771611834No Hit
GCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAA765170.10580204259044859Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATC750940.10383442354361969No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA748060.10343619846597617Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCG738620.10213090515592241No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)