FastQCFastQC Report
Thu 29 Jun 2023
SRR1646668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1646668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870830
Total Bases49.6 Mbp
Sequences flagged as poor quality0
Sequence length57
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAA36062841.41198626597614No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC583776.703604607098974No Hit
CACCACTGACTACACTGTAGTCACTGAATATACTACTTACTGTCCAGAAC242422.783780990549246No Hit
ACTTTTTCTTCGTTTTTAATATCCCGTAATTGATAAATCATGAAACTTTC197382.266573269179978No Hit
GTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAAT129531.4874315308383956No Hit
TTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATA124401.4285222144390983No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC124251.42679971980754No Hit
TATTGACTACCTCTAAAAACACAACCGAACTCTTGGATGGGTTTAGCACA84400.9691903126901922No Hit
GCCTCTTTTTCCCCCAACCAATTTATCATTATACACAAGTTCTACAACTA72080.827716086951529No Hit
AGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAA54330.6238875555504518No Hit
TAACTGCTTGAATAAGATACTACCAAAGGGAGAATTTGACCTGGAAGAAG50900.5844998449754832No Hit
TGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAG34430.39536993443037105No Hit
GCCCCAGACTCAATAATTCCTTCATCTAGCGCCTCCATCTCTGGTGTCTC27420.3148720186488752No Hit
AGAATTCTCCAGATGGTTCAACCACATCGCTTCCAAGGCCGATGAATTCG24220.2781254665089627No Hit
TATTAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAA23010.2642306764810583No Hit
GTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCT20640.2370152613024356No Hit
TAGAATAAGAAGAAGGACTAGCTCACCAGTAAGAACTGATAAATTTGCAT18580.21335966836236697No Hit
TGGCTTTAGACGACTTATCAATTGTTGTTATTGACGCAGTTACTCAAAGA16420.18855574566792602No Hit
TTAAAGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATA15130.1737422918365238No Hit
TCTGGCTCAGTAACTATCACATCTTCTGAAGCTCCAGAATCCGACAACGG14110.16202932834192665No Hit
GAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAAT13990.16065133263667994No Hit
GTTTTACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAAT13360.15341685518413467No Hit
GTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATA12600.14468954905090545No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG11680.13412491531068063No Hit
ATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGAAGACTAACTACTG11620.13343591745805725No Hit
ATATTAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGA11120.12769426868619593No Hit
AACCTCCTACCGAATTATCAGACTCCTTTAAAGAACTCCCACCGATAAAT10660.12241195181608351No Hit
CGGCTCAGTTGAACGTATATGATCACGATCGGCAAAATTCGATAGTCGAT10270.11793346577403166No Hit
TTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAG9940.11414397758460319No Hit
ACTACATCCTGAGCAACCGAGAAATGTCCTTTCAAAAGCTGTGAGTCCAA9760.11207698402673312No Hit
TTTAATGATGAAGATTTTAATTTTCCGTTGGTCTATTAAGAACAGAAGTA9640.11069898832148639No Hit
CTAAAACTTGTGGCGCCGTTCCACCACCACCACCGCCACCGCCTTTACCC9120.10472767359875061No Hit
AACGCTAGGTCACTCCTTACAGTCCGCGTCTTTTTCTAGTATTTTCCAAC9020.10357934384437836No Hit
TATTTACTACCTCTAAAAACACAACCGAACTCTTGGATGGGTTTAGCACA8710.10001952160582434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)