FastQCFastQC Report
Sat 2 Sept 2023
SRR16492701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16492701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22674498
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length36-97
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG12143295.355483503978787No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCATAACCCAGAGGTCGAT8483263.7413220791040223No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGT3991621.7604006051203425No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG3254281.435215897613257No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCCATAACCCAGAGGTCGAT3212951.4169883716940503No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTAATCCCAGGGTCGT2840751.2528392028789348No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTCACCCAGGCGGCCCG2650871.1690975473856136No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG2312821.0200093514749478No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGG1936490.8540387531402018No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTTCACCCAGGCGGCCCG1930570.851427890487366No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCATAACCCAGAGG1738580.7667556741498753No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTTCCAAGCAGTTGACCCG1697860.748797172929694No Hit
GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTTACACGCAGAAGGTCCT1435410.6330503987342961No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGGGT1221930.5389005745573727No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG1169280.5156806558628112No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTTCAGGTCGCAGTCTCCCTT899750.39681143106233263No Hit
AGCTCGCTCTGAAGGCCTGTTTCCTAGGCTACATACGAGGGACATGTTCC897930.39600876720622435No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACACGCGAAAGGTCCC892300.3935258015414497No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCCCATAACCCAGAGGTCGAT877700.3870868497287129No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGG814330.359139152716854No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCCCATTCTTGCGACCCGG810360.35738828705270564No Hit
CACCCCTGGCAATGGGCAAAGAGGCACCTTGTTTGTGGTGTTGTTCTTAT696580.3072085653230338No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGATTGAGGCTCCAGTCTCTT681430.3005270502570774No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGG654400.2886061689215788No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGG633410.2793490731305275No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCCATAACCCAGAGG632240.278833074937315No Hit
GCGTTGTGGTATAGTGGTGAGCATAGCTGCCTTCCAAGCAGTTGACCCGG629150.277470310478318No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG626760.27641626288705484No Hit
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCCCATAACCCAGAGGTCGAT612780.27025074601431087No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACACGCGAAAGGTCCC611290.26959362011013427No Hit
GCGTTTGTGGTATAGTGGTGAGCATAGCTGCCTTCCAAGCAGTTGACCCG562090.24789523454940435No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC490330.21624734536570558No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGG480600.21195618090420346No Hit
GTCCCCTTTTAAAAGCACTCAGTGAGCCTTTTGCTAGTGACCCACTGAGC479590.21151074656647306No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTAATCCCAGG477230.21046992969811282No Hit
TCCCCATGAAGAAATGTTCACACGTCCTACTTCCTGTCCTAGCTCCAGAG436110.19233501883922635No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCCCATAACCCAGAGGTCGATG427040.1883349302815877No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTCACCCAGGCG422870.18649585979808683No Hit
TTCCCCATGAAGAAATGTTCACACGTCCTACTTCCTGTCCTAGCTCCAGA421810.18602837425551824No Hit
TTGCAATGATGTTGTAATTTGCGTCTTACTCTGTTTTCAGTGACAGTTGC413070.18217382364981136No Hit
GGTGAATATTGTTTACAAAAAACATTAGACTGTGAATCTGACAACAGGAA412630.1819797730472357No Hit
CAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCTC390160.17206996159297552No Hit
CTTCGATGAAGAGGTGATGACGAGTCTGAGTAGGAAGTGTTGTCTTTGTC388830.171483399544281No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTCTGCGTTCAGGTCGCAGTCTCCC381860.16840946158984424No Hit
GTGCATGGGTTTGGATTTATGGCAGGCCCGTTCCCCTGGG376550.16606762363603375No Hit
AAGAAAGATTGCAAGAACTGCTAATTCATGCTTCCATGTTTAAAAACATG374540.16518116520154053No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCG371010.16362435013996782No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCATAACCCAGAGGT369470.1629451730309531No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG365900.1613707170055099No Hit
TCCCTGTTGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG364000.16053277122166057No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTTCCAAGCAGTTG360440.15896272543718498No Hit
GGTGAATATGGTTTACAAAAAACATTAGACTGTGAATCTGACAACAGGAA356410.15718539832723089No Hit
TCCTCCTTGGTATAGTGGTGAGTATCCCCGCCTGTCACGCGGGAGACCGG330020.14554677241366049No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTCACGCGGGAGACCGG318200.14033386758992417No Hit
TCCCACATTGTCTAGCGGTTAGGATTCCTGGTTTTCACCCAGGCGGCCCG315100.13896669288995944No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTCTGAATCCAGCGATCCG311450.13735695493677522No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTGGGGTGCGAGAGGTCCCG311000.13715849409323197No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTTCACCCAGGCG305310.1346490669826516No Hit
AAAGGTTTGGTCCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGC303200.1337185061384821No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCTTCAAACCTGTAGCTG301110.13279676577624783No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCATACCCCGAAAACGTTGGT292600.1290436507127964No Hit
TCCTCTTTTGTATAGTGGTGAGTATCCCCGCCTGTCACGCGGGAGACCGG267370.11791661275147083No Hit
ACACCCCTGGCAATGGGCAAAGAGGCACCTTGTTTGTGGTGTTGTTCTTA261340.11525723744799111No Hit
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC261050.11512934045992992No Hit
ACCCCCTGGCAATGGGCAGAGAGACACCTTGTTAATGGTGTTGTTCTTAT260970.11509405853218889No Hit
TGGGCCTCTTGTCCATTGAGCTGTGGGGATCGTGTTCCTCCATGGCCAGG260410.11484708503800173No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCATAAC250460.11045889527521181No Hit
CACAATGATGAACAATACTGCTGGAGCCACAGAGAGGCACAAGTGTGTGT250240.11036186997392401No Hit
TCCCATATTGTCTAGCGGTTAGGATTCCTGGTTTTCACCCAGGCGGCCCG246930.10890208021363913No Hit
GCCAATGATGACACCACACTAACTGAGCAGTTAGTAGTTGGTCCTTTGAT238510.10518865731889632No Hit
GCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGT238470.10517101635502582No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC236840.10445214707730244No Hit
AGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGA230530.10166928502672914No Hit
GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTTACACGCAGAAG228220.10065051936320707No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)