FastQCFastQC Report
Sat 2 Sept 2023
SRR16492702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16492702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39141510
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATGC651803116.652477127223758RNA PCR Primer, Index 35 (100% over 50bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATG11008612.812515408833231RNA PCR Primer, Index 35 (100% over 49bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATGCC8321222.1259322902974365RNA PCR Primer, Index 35 (100% over 50bp)
GTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATCTGGAATTCTCGG6344981.6210360816432476No Hit
AATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTAT6098781.5581361066550574RNA PCR Primer, Index 35 (100% over 48bp)
AAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTA6068381.5503694159985142RNA PCR Primer, Index 35 (100% over 47bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATG3669780.9375673038674287RNA PCR Primer, Index 35 (100% over 49bp)
TCTACAGTCCGACGATCTCTACAGTCCGACGATCTGGAATTCTCGGGTGC2187540.5588798183820706No Hit
GTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATTGGAATTCTCGGG2003370.5118274690986628No Hit
AACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTA1833750.468492401034094RNA PCR Primer, Index 35 (100% over 47bp)
TCTACAGTCCGACGATCGTTCTACAGTCCGACGATTGGAATTCTCGGGTG1735190.44331197237919534No Hit
TCTACAGTCCGACGATCTTCTACAGTCCGACGATCTGGAATTCTCGGGTG1532700.391579170042239No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTAT1481500.3784984278838502RNA PCR Primer, Index 35 (100% over 48bp)
GTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATCATGGAATTCTCG1421510.363171987999441No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGGTGC1359710.3473831234410732No Hit
TACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTA1264710.32311221513937505RNA PCR Primer, Index 35 (100% over 47bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1221280.3120165777968198RNA PCR Primer, Index 1 (100% over 31bp)
TTCTACAGTCCGACGATCGTTCTACAGTCCGACGATTGGAATTCTCGGGT1137820.2906939461456648No Hit
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1087500.2778380292431232RNA PCR Primer, Index 1 (100% over 33bp)
GTTCTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCT1087300.277786932594067RNA PCR Primer, Index 35 (100% over 43bp)
GATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTA1056030.26979797151412915RNA PCR Primer, Index 35 (100% over 47bp)
GATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGT1039250.2655109626583134RNA PCR Primer, Index 35 (100% over 46bp)
TCTACAGTCCGACGATCCTACAGTCCGACGATCTGGAATTCTCGGGTGCC1031440.2635156385126685No Hit
ATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTA1029500.2630200010168233RNA PCR Primer, Index 35 (100% over 47bp)
ACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTAT975120.24912682213844073RNA PCR Primer, Index 35 (100% over 48bp)
GTTCTACAGTCCGACGATCTCTACAGTCCGACGATCTGGAATTCTCGGGT863870.22070431110092584No Hit
CTACAGTCCGACGATCGTTCTACAGTCCGACGATTGGAATTCTCGGGTGC846800.2163432121039786No Hit
TCTACAGTCCGACGATCGTTCTACAGTCCGACGATCTGGAATTCTCGGGT814910.20819585141196648No Hit
TTCTACAGTCCGACGATCTCTACAGTCCGACGATCTGGAATTCTCGGGTG791530.20222265313729593No Hit
GTTCTACAGTCCGACGATCATGGAATTCTCGGGTGCCAAGGAACTCCAGT762900.19490816782489995RNA PCR Primer, Index 1 (100% over 30bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATG748760.19129563473662617RNA PCR Primer, Index 35 (100% over 49bp)
GTTCTACAGTCCGACGATCTTCTACAGTCCGACGATCTGGAATTCTCGGG717670.18335266064083883No Hit
TTCTACAGTCCGACGATCGTTCTACAGTCCGACGATCTGGAATTCTCGGG702600.1795025281344537No Hit
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA681250.17404796084770363RNA PCR Primer, Index 1 (100% over 32bp)
CTACAGTCCGACGATCTCTACAGTCCGACGATCTGGAATTCTCGGGTGCC681160.17402496735562833No Hit
TCTACAGTCCGACGATCTCTACAGTCCGACGATTGGAATTCTCGGGTGCC657100.16787804047416668No Hit
CAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTA640980.16375965056023642RNA PCR Primer, Index 35 (100% over 47bp)
TTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATG631590.16136066288704753RNA PCR Primer, Index 35 (100% over 49bp)
GTTCTACAGTCCGACGATCGTTCTACTGGAATTCTCGGGTGCCAAGGAAC622260.1589770042085755RNA PCR Primer, Index 1 (100% over 24bp)
TCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTA593750.15169317688561326RNA PCR Primer, Index 35 (100% over 47bp)
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTTGGAATTCTCGGGTG573640.14655540882301168No Hit
GTTCTACAGTCCGACGATCAGTTCTACAGTCCGACGATCTGGAATTCTCG569760.14556413383132127Illumina DpnII expression Sequencing Primer (95% over 21bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC560100.1430961656819065No Hit
GTTCTACAGTCCGACGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA554000.1415377178856922RNA PCR Primer, Index 1 (100% over 32bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC553610.1414380794200326RNA PCR Primer, Index 2 (100% over 34bp)
TTCTACAGTCCGACGATCTTCTACAGTCCGACGATCTGGAATTCTCGGGT546770.13969057402231033No Hit
TACAGTCCGACGATCGTTCTACAGTCCGACGATTGGAATTCTCGGGTGCC537250.1372583735272349No Hit
TCTACAGTCCGACGATCGTTCTACAGTCCGACGATCATGGAATTCTCGGG524980.13412359410763663No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCTGGAATTCT516050.131842128727277No Hit
CTACAGTCCGACGATCGTTCTACAGTCCGACGATCTGGAATTCTCGGGTG513650.13122896893860253No Hit
GTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTA496050.12673246382165634RNA PCR Primer, Index 35 (100% over 47bp)
TCTACAGTCCGACGATCTACAGTCCGACGATCTGGAATTCTCGGGTGCCA494400.12631091646694265No Hit
CCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTAT488380.12477290733035082RNA PCR Primer, Index 35 (100% over 48bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA485120.12394003195073465RNA PCR Primer, Index 1 (100% over 28bp)
AAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGT473740.1210326326194365RNA PCR Primer, Index 35 (100% over 46bp)
CCGACGATCGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGA472180.12063407875679809RNA PCR Primer, Index 1 (100% over 22bp)
TCTACAGTCCGACGATCTCTACAGTCCGACGATCATGGAATTCTCGGGTG469840.12003624796284046No Hit
TCTACAGTCCGACGATGTTCTACAGTCCGACGATCTGGAATTCTCGGGTG456260.11656678549192405No Hit
GTTCTACAGTCCGACGATCCTACAGTCCGACGATCTGGAATTCTCGGGTG446760.11413969466175425No Hit
TACAGTCCGACGATCTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCA432330.11045307143234893No Hit
TCTACAGTCCGACGATCAGTTCTACAGTCCGACGATCTGGAATTCTCGGG421150.10759676875010699Illumina DpnII expression Sequencing Primer (95% over 21bp)
AATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTA416810.1064879714655873RNA PCR Primer, Index 35 (100% over 47bp)
TCTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCG410950.10499083964824044RNA PCR Primer, Index 35 (100% over 45bp)
GTTCTACAGTCCGACGATGTTCTACAGTCCGACGATCTGGAATTCTCGGG395620.10107428149808222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)