FastQCFastQC Report
Sat 2 Sept 2023
SRR16492703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16492703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58444102
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG15075012.5793894480575643No Hit
AGAGTTCTACAGTCCGACGATCGTTCTACTGGAATTCTCGGGTGCCAAGG13325062.279966590982953Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTCCGACGATCGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAA9927651.6986572913721902No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGC5750400.9839145103127771No Hit
AGTCCGACGATCGTTCTACAGTCCGACGATCATGGAATTCTCGGGTGCCA5155290.8820890087420626No Hit
AGAGTTCTACAGTCCGACGATCTTCTACTGGAATTCTCGGGTGCCAAGGA5095370.8718364771863549Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC4332750.741349400834322No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT3762340.6437501597680464No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAATGGAATTCTCGGGTGCCAA3132370.5359599844651561No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGTGGAATTCTCGGGTGCCA3062070.5239313968755992No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGATGGAATTCTCGGGTGCC3059410.5234762611289673No Hit
AGTCCGACGATCGTTCTACAGTCCGACGATTGGAATTCTCGGGTGCCAAG2912670.49836850945198885No Hit
AGAGTTCTACAGTCCGACGATCGACGATCTGGAATTCTCGGGTGCCAAGG2410110.4123786519981092Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAGTCCGACGATCTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAA2129550.3643738079849358No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC2038720.34883246217043423No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTATGC1814370.3104453551189819RNA PCR Primer, Index 28 (100% over 50bp)
TGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTTGGAATTCTCGG1778780.3043557757119786No Hit
GTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTTGGAATTCTCG1599610.2736991322067024No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAA1593330.2726246011958572No Hit
ACAGTCCGACGATCGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCC1545240.26439622598701235No Hit
AGTCCGACGATCGTTCTACAGTCCGACGATGGAATTCTCGGGTGCCAAGG1507410.25792337437231905Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGG1440830.24653129241339017No Hit
AGAGTTCTACAGTCCGACGATCAGTTCTACTGGAATTCTCGGGTGCCAAG1400410.23961528230855528Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA1389610.23776736273576418No Hit
AGAGTTCTACAGTCCGACGATCTCTACAGTCCGACGATCTGGAATTCTCG1364030.23339053100687562Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAATGGAATTCTCGGGTGCCA1337800.22890248189629125No Hit
AGAGTTCTACAGTCCGACGATCGTTCTTGGAATTCTCGGGTGCCAAGGAA1291570.22099235950276044RNA PCR Primer, Index 1 (100% over 23bp)
AGAGTTCTACAGTCCGACGATCTCTACTGGAATTCTCGGGTGCCAAGGAA1211410.20727668978471084RNA PCR Primer, Index 1 (100% over 23bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA1176230.201257262880008RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGGT1159110.19832796814980577No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA1121400.19187564897481013No Hit
CCGGGCCGCCCCTCCCACGGCGCGACCGCTCTTGGAATTCTCGGGTGCCA1059790.18133395222669346No Hit
AGAGTTCTACAGTCCGACGATGTTCTACTGGAATTCTCGGGTGCCAAGGA1037290.17748411978337866RNA PCR Primer, Index 1 (100% over 22bp)
AGTCCGACGATCGTTCTACAGTCCGACGATCAATGGAATTCTCGGGTGCC1007340.1723595650421663No Hit
CGAGTTCTACAGTCCGACGATCCCGACGATCTGGAATTCTCGGGTGCCAA989330.16927798805087294Illumina DpnII expression Sequencing Primer (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC964410.16501408474032161No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGGT961220.16446826405169165No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCCCCGTGGAATTCTC923750.15805700975609138No Hit
GGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTTGGAATTCT918580.1571724038124497No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCA904320.15473246556170886No Hit
AGTCCGACGATCTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGG870110.14887900921122887Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGTTCTACAGTCCGACGATCTCCGACGATCTGGAATTCTCGGGTGCCA843840.14438411595407866Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAGTTCTACAGTCCGACGATCAAAAATGGAATTCTCGGGTGCCAAGGAA813030.13911241206169955RNA PCR Primer, Index 1 (100% over 23bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC807800.13821753989820906No Hit
AGTCCGACGATCGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCC762150.13040665762988368Illumina Small RNA Adapter 1 (95% over 22bp)
AGAGTTCTACAGTCCGACGATCAGTCCGACGATCTGGAATTCTCGGGTGC746470.12772375217605364Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG743800.12726690539278027No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCTGGAATTCTCGGGTGCCAAGGAA743340.1271881977072725RNA PCR Primer, Index 1 (100% over 23bp)
CGAGTTCTACAGTCCGACGATCCGACGATCTGGAATTCTCGGGTGCCAAG738070.1262864813972161Illumina DpnII expression Sequencing Primer (95% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATTGGAATTCTCGGGTGC708140.12116534872928666No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT694150.1187716084678656No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCTGGAATTCT689490.11797426539293905No Hit
CTACAGTCCGACGATCTCTACAGTCCGACGATCTGGAATTCTCGGGTGCC684580.11713414640197568No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAATGGAATTCTCGGGTGC667620.11423222825803707No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC667460.1142048516717735No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGTGGAATTCTCGGGTG643970.11018562660095282No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTTGGAATTC639520.10942421529549723No Hit
ACAGTCCGACGATCGTTCTACAGTCCGACGATTGGAATTCTCGGGTGCCA614740.1051842664979265No Hit
AGAGTTCTACAGTCCGACGATCAAGTTCTACTGGAATTCTCGGGTGCCAA603680.1032918599724571Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCT599780.10262455568228253RNA PCR Primer, Index 28 (100% over 43bp)
AGAGTTCTACAGTCCGACGATCGATCATGGAATTCTCGGGTGCCAAGGAA588390.1006756849476445RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)