FastQCFastQC Report
Mon 18 Sept 2023
SRR16584066.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16584066.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4558
Total Bases8.6 Mbp
Sequences flagged as poor quality0
Sequence length1-5543
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAG150.32909170688898637No Hit
CCTGCCAGTAGTCAACTCGCTTGTCAAAGATTAAGCCATGCATGTCTAAG110.2413339183852567No Hit
AGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGA100.2193944712593243No Hit
AGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAGAA90.19745502413339183No Hit
CCTGCCAGTAGTCAACTCGCTTGTCTCAAAGATTAAGCCATGCATGTCTA80.17551557700745943No Hit
CCTGCCAGTAGTCATCGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG70.153576129881527No Hit
TCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAA60.13163668275559456No Hit
CAGGTGGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAG60.13163668275559456No Hit
CCTGCCAGTAGTCATCGCTTGTCAAAGATTAAGCCATGCATGTCTAAGTA60.13163668275559456No Hit
AGGTAGGAGTACCCGTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA60.13163668275559456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)