FastQCFastQC Report
Mon 18 Sept 2023
SRR16584068.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16584068.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length3-7417
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGTCATCGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG140.472972972972973No Hit
AGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGA110.37162162162162166No Hit
CCTGCCAGTAGTCATCGCTTGTCAAAGATTAAGCCATGCATGTCTAAGTA90.30405405405405406No Hit
CAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAG80.2702702702702703No Hit
TCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAA80.2702702702702703No Hit
CCTGCCAGTAGTCAACTCGCTTGTCAAAGATTAAGCCATGCATGTCTAAG70.2364864864864865No Hit
CCTGCCAGTAGTCAACTCGCTTGTCTCAAAGATTAAGCCATGCATGTCTA60.20270270270270271No Hit
CAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAGA30.10135135135135136No Hit
CCTGCCAGTAGTCAACATGCTTGTCTTGAGATTAAGCCATGCATGTCTAA30.10135135135135136No Hit
CCTGCCAGTAGTCAACTTGCTTGTCAAAGATTAAGCCATGCATGTCTAAG30.10135135135135136No Hit
CAGGTAGGAGTACCCGCTGAACTTAAGCATCAATAAGCGGAGGAAAAGAA30.10135135135135136No Hit
CCTGCCAGTAGTCAACTTGCTTGTCTCAAAGATTAAGCCATGCATGTCTA30.10135135135135136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)