FastQCFastQC Report
Mon 18 Sept 2023
SRR16584074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16584074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3979
Total Bases5.3 Mbp
Sequences flagged as poor quality0
Sequence length2-6625
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGA80.20105554159336514No Hit
CCTGCCAGTAGTCAACATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA50.12565971349585323No Hit
CCTGCCAGTAGTCATCGCTTGTCAAAGATTAAGCCATGCATGTCTAAGTA50.12565971349585323No Hit
CCTGCCAGTAGTCAACTCGCTTGTTCAAAGATTAAGCCATGCATGTCTAA40.10052777079668257No Hit
CAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAG40.10052777079668257No Hit
CCTGCCAGTAGTCAACATGCTTGTCAAAGATTAAGCCATGCATGTCTAAG40.10052777079668257No Hit
AGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAGAA40.10052777079668257No Hit
CCTGCCAGTAGTCAACTCGCTTGTCAAAGATTAAGCCATGCATGTCTAAG40.10052777079668257No Hit
AGGTAGGAGTACCCGCTGAACTTCTAAGCATATCAATAAGCGGAGGAAAA40.10052777079668257No Hit
CAGGTAGGAGTACCCGCTGAACTTAAGCATATCAAATAAGCGGAGGAAAA40.10052777079668257No Hit
CCTGCCAGTAGTCATCGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG40.10052777079668257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)