FastQCFastQC Report
Mon 18 Sept 2023
SRR16584077.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16584077.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22536
Total Bases37.7 Mbp
Sequences flagged as poor quality0
Sequence length1-21151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAG660.2928647497337593No Hit
AGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGA600.26624068157614483No Hit
CCTGCCAGTAGTCAACTCGCTTGTCTCAAAGATTAAGCCATGCATGTCTA590.26180333688320906No Hit
CCTGCCAGTAGTCATCGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG590.26180333688320906No Hit
CCTGCCAGTAGTCATCGCTTGTCAAAGATTAAGCCATGCATGTCTAAGTA430.19080582179623712No Hit
TCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAA410.18193113241036563No Hit
CCTGCCAGTAGTCAACTCGCTTGTCAAAGATTAAGCCATGCATGTCTAAG340.1508697195598154No Hit
AGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAGAA270.11980830670926518No Hit
CCTGCCAGTAGTCAACTTGCTTGTCTCAAAGATTAAGCCATGCATGTCTA250.11093361732339369No Hit
AGGTGGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGA240.10649627263045794No Hit
CCTGCCAGTAGTCAACATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA240.10649627263045794No Hit
CAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAGA230.10205892793752219No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)