FastQCFastQC Report
Tue 19 Sept 2023
SRR16796941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72562320
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC29040214.002106051735942Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT22077033.042492301789689Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC16356402.254117564047015Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC12271201.6911256420687761Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT10231341.4100072875288443Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT8649781.1920484350555494Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT8580901.18255590504824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT8334811.1486416090334488No Hit
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA7226880.9959549253662232Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG6275240.8648069686856761Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG5564690.7668842451564393Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTCCAG5526200.7615798392333651Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC5520830.7608397857179869No Hit
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG4810160.6629005246800268Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATG4671030.6437266614408139TruSeq Adapter, Index 1 (97% over 37bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT4494150.619350373582322Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG4143880.5710787637440479Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT4134720.5698164005781513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC4034510.5560062026682718No Hit
TGTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTC3737230.5150372810571657No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3649050.5028849683968208Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC3609510.4974358592724158Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA3591310.49492767044934616Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTCT3201230.44116974209203896Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2976540.4102046351329451No Hit
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC2907780.40072864263435903No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2786240.3839789025488711No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2728970.3760863765105636No Hit
GCGCAGCGGAAGCGTGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCA2475920.3412129049898074Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC2437040.3358547521633818No Hit
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA2338370.3222567856154544Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT2311420.31854273678129363No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG2236840.3082646751096161Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT2143480.29539849332270524Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC2030650.27984910074540065No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1983730.27338293483449816Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1852000.2552288846332366No Hit
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG1798310.24782972760518132Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1758820.24238750910941104No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1728470.23820489752808344Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA1664090.22933252409790644Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1581290.2179216430786667Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1553940.2141524692154275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1548060.21334213128797425No Hit
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG1490280.20537932083759175Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1482630.20432505465646633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAAC1479570.20390334818401615Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAG1478040.20369249494779107Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGT1457640.20088111846478998TruSeq Adapter, Index 1 (97% over 34bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT1403790.1934599114251033Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1385390.19092416008749447Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1374620.1894399186795571Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1354820.18671122974017368Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA1284710.17704919026844787Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT1282350.176723952596885No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC1235250.17023298042289717Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTCTG1234990.17019714915399617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA1205590.1661454595167299Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGTAGTGTAGTGGTTATCACGTTCGAGATCGGAAGAGCACACGTCTGA1152710.15885793067255843Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCA1087190.14982845090950786Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT1078570.1486405065328672Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCCAGATCGGAAGAGCACACGT1067240.14707909008422002No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA1048790.1445364481179764Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAACTCCAG1040840.14344083816504213Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGATACCCTTTATGAAATTAAAGGACAGATCGGAAGAGCACACGTCTGA1039010.14318864115700822Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGGAGATCGGAAGAGCACACGTCTGAACTCCA1002050.13809508847015917Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC999610.1377588257927806Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCGCTGGTGTAGTGGTATCAGATCGGAAGAGCACACGTCTGAACTCCA998980.13767200387198203Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT993230.13687958157897928Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA992840.1368258346756278Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGT990620.1365198907642424No Hit
AGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTC985730.1358459872837583Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG983390.13552350586364934Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCAATGGATAGCGCATTGGAGATCGGAAGAGCACACGTCTGAACTCCA957340.1319334883449151Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAG953990.13147181622638304Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC946960.13050299384032923Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGCGGTTAGGATTCCTAGATCGGAAGAGCACACGTCTGAACTCCAG937200.129157943130815Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTGA930640.1282538926539284Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA900940.12416085924485325Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC890320.12269728972282032Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC880500.12134397025894432Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTG856890.11809021541758864Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC835080.11508452320708598No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC825860.11381389128682766Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA815840.11243300930841242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT795990.10969742974039419No Hit
TTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAA787300.1084998384836648Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCA766890.10568708387493674Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA763900.1052750242825753Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC756220.10421662372426901Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGTAATGGTTAGCACTCTGGAAGATCGGAAGAGCACACGTCTGAACTCC755470.1041132642947469Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT754410.10396718296768902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGT754050.10391757044151839No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)