FastQCFastQC Report
Tue 19 Sept 2023
SRR16796942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55162304
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT17262443.1293906795481203Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT13806332.50285593582168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC13660372.476395837273222Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC12603662.284832047624407Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC11117952.015497757309049Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG9673231.7535942661133226Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT8765231.5889891038633919Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG6784941.2299957594229567Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA4740560.8593839735193077Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG3763220.6822086329098944TruSeq Adapter, Index 5 (100% over 49bp)
GTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTCT3641230.6600938931049726Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT3498170.6341595158896917No Hit
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG3343830.6061802639715701Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTC3247830.5887770750112251No Hit
CTCGGTTGGCCCCGGATAGCCGGTTCCCCGAGATCGGAAGAGCACACGTC3044980.5520037741715792No Hit
ACGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAAC2541830.4607911228653538Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC2372510.4300962483365452Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2363360.4284375068887623No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2325240.42152699060575866No Hit
CCTCGGTTGGCCCCGGATAGCCGGTTCCCCGAGATCGGAAGAGCACACGT2316850.4200060244039118No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG2290510.4152310244329171Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT2102590.38116428204304154Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA2045860.37088008506678766Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT1932020.35024280349131176Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC1896280.34376374126794995Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1861800.33751309589969264No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1667570.3023024564021111Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG1637580.2968657726841867No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1629860.29546626623862554Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA1519230.27541090379401123Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTCT1455400.2638395959675651Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1384450.25097755162656005No Hit
GTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGTC1259530.228331651991911No Hit
CTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTCCA1242020.22515738283883138Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1222670.22164955256401184Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1217680.22074494930451055No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCCAGATCGGAAGAGCACACGT1202340.2179640647352221No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1146040.20775781954285302Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1102170.19980492475441203Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC1099230.19927195209250145Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT1087300.1971092432977419Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT1078420.19549944831890997Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA1016080.18419825248778587Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT993390.18008493626372096No Hit
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC963880.17473526849059823No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC958870.1738270395667302No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT936330.16974091582541584Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC934540.16941641886459274Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA898790.16293554380904757No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC896220.16246964593792165No Hit
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA867990.1573520206842702Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC860060.15591444476285835No Hit
TGAGTCTGAAACCAATTTTTTGAGGCCTTGAGATCGGAAGAGCACACGTC844260.1530501699131349No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC840370.15234497819380424No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT833230.15105061601487857Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT819280.1485217151190784Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCTGAACTCCAG792850.14373039965843343Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATCCGTACTATTAACCCCAAGATAAGGGTTTAAGATCGGAAGAGCACAC773080.14014643043191233No Hit
TCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTC770400.13966059140676937Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC767600.13915299839542597No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC764740.13863452839098236No Hit
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC742410.13458647412551877Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC727780.1319343006412495Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT727740.13192704931251603No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT724640.13136507133567155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT720500.13061455881175665TruSeq Adapter, Index 5 (100% over 50bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA716680.1299220569177096Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG709540.12862769473878394No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC695600.12610060667516715Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC682690.12376024032643741Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT675890.12252751444174631No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT674050.12219395332000635No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT665670.1206747999503429Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACAC665490.12064216897104225No Hit
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC661180.1198608383000101Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG650910.11799905964768984No Hit
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC641290.1162551150872886Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC638510.11575114774031194No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC615440.11156894389327901No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA608610.11033077951203779Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC607830.11018937860173497No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT605390.10974704754899288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGCACTGAAAATGCTTAGATGAGATCGGAAGAGCACACGTCTGAACTC604000.10949506387550455Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC603160.10934278597210152No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG600610.10888051376534236Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT591290.1071909541704422No Hit
ATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACGTCTGAACTC585770.10619027080522234Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC584760.10600717475470205Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA573720.10400580802426238Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGTTCCCAGATCGGAAGAGCACACGTCT563540.10216034486159244Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAGTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACT560850.10167269300426611Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAACTCC554070.10044359278394172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)