FastQCFastQC Report
Tue 19 Sept 2023
SRR16796951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65380926
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length10-67
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT58439658.938333176865681No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC11903011.8205630798193346No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGG7211861.1030525936570552No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC6781601.0372444097839788No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC6672741.0205942938159058No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC6397920.9785606279115717No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC5084660.7776977646355147No Hit
CGCCGTCGGGCCCGGGGGGCCGGTCACGCCG4949920.7570893076674993No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT4735940.7243611080087792No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC4703350.7193764738052196No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT4527970.6925521366889175No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACG4142810.6336419891024486No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG3444300.5268050195557035No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC3281980.5019782069161884No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC2914520.4457752709100511No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2717830.4156915734108752No Hit
AAGCCGCCGTGGCGCAATGAAGGTGAAGGGCC2635020.4030257999099003No Hit
AGCCGCCGTGGCGCAATGAAGGTGAAGGGCC2311070.35347770999756106No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGA2186310.3343956921013936No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2047300.31313413945834906No Hit
GGCCGCCGGTGAAATACCACTACTCTCAT1988030.3040688044094083No Hit
GGCTCCATAGCTCAGGGGTTAGAGCACTGG1600590.2448099312634391No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGG1574200.24077358586202954No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT1523690.23304809112064273No Hit
GGCCGCCGGTGAAATACCACTACTCTCA1384550.21176665500271438No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGA1363710.20857918102903591No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1294810.19804093934062666No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1257380.1923160280721628No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGG1146640.17537836646730884No Hit
CGCCGTCGGGCCCGGGGGCCGGTCACGCCG1115090.17055280006281953No Hit
AAAGCCGCCGTGGCGCAATGAAGGTGAAGGGCC1094290.16737144408141297No Hit
AGCCGCCGTGGCGCAATGAAGGTGAAGGGC1082520.1655712248553959No Hit
GGCGGAAGCCGAGCGCACGGGGTCGGCGGCGA1080100.1652010863229438No Hit
TACCCGTCGCCGCGCTCTCCCCCACCG1002480.15332912231925255No Hit
AAGCCGCCGTGGCGCAATGAAGGTGAAGGGC995080.15219729374894445No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC971920.1486549762234937No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA962520.1472172480395888No Hit
GGGCGGAAGCCGAGCGCACGGGGTCGGCGGCGA962280.14718054008595718No Hit
ATTGATCATCGACACTTCGAACGCACT943450.14430049522394345No Hit
AAAGCCGCCGTGGCGCAATGAAGGTGAAGGGC926500.14170799599871067No Hit
AGATGGGCGAGCGCCGTTCCGAAGGGACGGGCG874640.13377601901814606No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG869130.1329332655826869No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAA794240.1214788545515553No Hit
AGATGGGCGAGCGCCGTTCCGAAGGGACGGGC784750.12002736088503854No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGG779590.11923813988195885No Hit
ACGCGTTAGGACCCGAAAGATGGTGAACT765890.11714272752882088No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG707940.10827928622485401No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA698340.10681096807958945No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG690700.10564243155564974No Hit
CTAAACCATTCGTAGACGACCTGCTT671210.10266143982114906No Hit

[OK]Adapter Content

Adapter graph