FastQCFastQC Report
Tue 19 Sept 2023
SRR16796956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57555741
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT20047423.4831312483666923Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG13461592.3388787575508756Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC12624672.1934684152533106Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT12433142.160191109345634Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC11312531.9654911575198033Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9338761.622559250866043Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG8830131.5341875278784092TruSeq Adapter, Index 3 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA7275791.2641293246489522Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT6759411.1744110809032933Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4151700.7213355136892425Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG3784710.6575729778198842Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC3752060.6519002161747861Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA3694280.6418612523814088Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG3617380.6285002915695239Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT3296810.5728029806791993TruSeq Adapter, Index 3 (100% over 50bp)
ATTCGAACGTCTGCCCTATCAGATCGGAAGAGCACACGTCTGAACTCCAG3146300.5466526788352877Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC2812880.48872274965585105Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC2673490.4645044879189376Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2659480.46207032587765656Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC2452680.4261399397151363No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2390280.41529827580536227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT2131650.3703627063024Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2106720.36603125307690854Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT2019060.3508008002190433TruSeq Adapter, Index 3 (100% over 35bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1935870.336346985785484Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1828020.3176086291721968Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1695820.29463959120950245Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG1482660.25760418930233214Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1426120.24778066883023886Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1354550.2353457668106471Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1348210.23424422595827582Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1329830.23105079995408276Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCG1232770.2141871477251939TruSeq Adapter, Index 3 (100% over 50bp)
TCGACTGCATAATTTGTGTTAGTGGGGAGATCGGAAGAGCACACGTCTGA1121350.1948285228401455Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACCCGGTGAGGCGGGAAGATCGGAAGAGCACACGTCTGAACTCCAG1064420.18493724196861613Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTC1059410.1840667814527833No Hit
ATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1052550.18287489340116395Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCGAACGTCTGCCCTATAGATCGGAAGAGCACACGTCTGAACTCCAGT976660.16968941464935705Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTTCTACAGTCCGACGATCCGGAAGAGCACACGTCTGAACTCCAGTCAC939550.16324175202609242Illumina Multiplexing Index Sequencing Primer (96% over 31bp)
ATTCGAACGTCTGCCCTATCAAAGATCGGAAGAGCACACGTCTGAACTCC872220.1515435271696007Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATAGCTAAAGACACCTTGCCTAGCCACGCAGATCGGAAGAGCACACGTC857040.14890608393001145No Hit
ATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG835420.14514972537665705Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA823330.1430491529941383Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA818220.14216131801691165Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA805420.13993738695849645Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTCTACAGTCCGACGATCATCGGAAGAGCACACGTCTGAACTCCAGTCA770200.13381810165557595Illumina Multiplexing Index Sequencing Primer (96% over 32bp)
ATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCA754520.13109378610901734Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA748460.1300408937485489Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA731940.12717063272628182Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA710020.12336215078874582TruSeq Adapter, Index 3 (100% over 36bp)
TAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC674200.11713861871746208Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCGAACGTCTGCCCTATCAAGATCGGAAGAGCACACGTCTGAACTCCA664020.11536989854756625Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA659470.11457936055414525Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG648210.11262299620119563Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTCTACAGTCCGACGATCCGGAAGAGCACACGTCTGAACTCCAGTCACT636480.11058497187969485TruSeq Adapter, Index 3 (96% over 32bp)
ATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACGTCTGAACTC635030.11033304218948377Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA590710.1026326808997212Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC581830.10108982872794565Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCAGATCGGAAGAGCACACGTCTGAACTCCAG577810.10039137537991213Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)