FastQCFastQC Report
Tue 19 Sept 2023
SRR16796957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73148426
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT27306743.7330591365014474Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC17802772.433787160368974No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG13279181.8153746739540233Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA11448341.5650835740471027Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT8799681.2029896583147257Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG7126250.9742178184394563TruSeq Adapter, Index 11 (100% over 49bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG6496090.888069689975284Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT5550380.7587832443585321Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4921060.6727499509011992Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG4556250.6228773808475386Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT4394120.6007128574441233No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG3928440.5370505169858337Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC3526940.4821621178834388Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT3168000.4330920257942393No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC3141240.4294337105763561Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT3111340.42534613116624Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGT2894290.39567358564899263TruSeq Adapter, Index 11 (100% over 50bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2678880.366225241811765Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2537500.3468974164939653Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA2221690.3037235551726021Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGTTAGTGGGGAGATCGGAAGAGCACACGTCTGA2141310.29273493868480505Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC2099260.2869863529257622No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1957060.2675464267679526Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC1885670.2577868182700199No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1779790.2433121390745988Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1548550.21169970219181478Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA1542500.21087261672588825Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA1486170.20317183585057594Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1466100.20042809943716355Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC1401340.19157486724321313Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC1387590.18969512754792564TruSeq Adapter, Index 11 (100% over 50bp)
AACTTTCGATGGTAGTCGATGTGCCTACCAGATCGGAAGAGCACACGTCT1341010.18332725300199898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1304460.17833056312107112No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT1282560.17533665044275867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT1275200.17433047704949933No Hit
TGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1232320.16846842336703186Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1213180.16585182571119164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGATGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA1189560.16262277468554143Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG1120490.1531803295398318No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1095050.14970246933269624No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1043050.14259363557597263No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC1032830.14119647632609347Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC1029990.14080822463630316Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG992960.1357459147514671No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT975090.13330293668930074No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG974740.1332550887697843Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC970370.1326576733175366No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC950790.1299809239914472Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT937060.1281039184629892Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG927380.1267805817175068Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC910060.12441279324315194Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA835340.114197945968106Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAAACCGAAGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC833960.11400928845686988No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT828610.1132778988299762No Hit
TGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG825200.1128117233855449Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG813190.11116985620442468Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC802680.10973305153551766No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTC783610.10712602346358074No Hit
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA761500.10410340203355845Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)