FastQCFastQC Report
Tue 19 Sept 2023
SRR16796958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61138204
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT13348242.183289518939745Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT9328891.5258691603044146Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC8537451.3964181872270898Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC8267391.3522461340212086Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG8195911.3405545900563256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT7947401.2999073378079604Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC7818111.2787601677013607Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT6850851.1205513985984932No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC6846691.1198709729844207No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATG5923510.968872098369131TruSeq Adapter, Index 7 (97% over 35bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA5172560.846043825559547Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT5011170.8196462558828191No Hit
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG4979900.8145316143078066Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC4015450.6567824596221374No Hit
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG3082870.5042460848212028Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC2803670.4585790580305565No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG2746420.4492150276445805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA2715000.4440758514921374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC2644410.43252987935334175No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG2608700.4266890142863863Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2602560.42568473225023096Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG2553770.41770445203133544Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC2444130.3997713115681318Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2258980.369487464826412Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2211050.361647849518118Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT2135690.3493216778170324Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA2109100.34497251505785154Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2051840.3356068490333802No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT2035720.3329701997788486Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT2005480.3280240289688588No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCCGT1986630.324940850405092RNA PCR Primer, Index 32 (97% over 34bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1933530.3162556099946934Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTCT1820540.2977745306355417Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1820110.29770419818024096No Hit
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1630050.26661725293729593Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1609400.2632396594443631Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1566070.25615243784393793Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA1526000.24959843439300244Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTC1482830.24253738300850317No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1476340.24147585362500998No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1386510.22678291302112835Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG1284000.21001598280512135No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1171500.19161504973224272No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1166570.19080867995402678Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT1158480.18948544841127488No Hit
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1117240.18274007525638142Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG1080610.17674873144785214Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCCAGATCGGAAGAGCACACGT1079070.17649684311956562No Hit
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA1069660.17495770729542529Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1052310.17211987450596355No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1021720.16711645634863595No Hit
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG1001980.16388770595878152Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG969500.15857515212582954Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC930730.15223378167929172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT895930.1465417597154146Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGTTAGTGGGGAGATCGGAAGAGCACACGTCTGA810830.13262247611984151Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAACCGAAGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT808480.1322381010734303Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG788950.12904369909197855Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC781660.12785131862885604Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA775390.1268257732922609Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCCG752000.12300001485159753TruSeq Adapter, Index 7 (96% over 33bp)
ACGATACCCTTTATGAAATTAAAGGACAGATCGGAAGAGCACACGTCTGA733610.11999207565861765Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC717190.11730635724922504Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC715540.1170364768974895No Hit
TGAGTCTGAAACCAATTTTTTGAGGCCTTGAGATCGGAAGAGCACACGTC689400.11276091787060019No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG680340.1112790293937977No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA647430.10589614310554493Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT645520.10558373615292985No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT640850.10481989297559345Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCCGTC630440.10311719330191643RNA PCR Primer, Index 32 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)