FastQCFastQC Report
Tue 19 Sept 2023
SRR16796959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62166403
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT35385875.6921211928571775Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC20656753.3228157015936723Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG16302182.6223457065707985Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC15713462.527645036821577Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC14901642.3970568153991474Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAAC9161601.4737220681724177Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT8913361.4337905315190909Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT7524111.210317733840898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT5353710.8611902477291471Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG5331260.8575789723590731Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC4747860.7637340703144752Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT4399950.7077697578867479No Hit
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3807130.6124095679140388Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3760440.6048990802958312Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC3657280.5883049080385108Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC3646040.5864968574746072Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC3540140.5694619326776877TruSeq Adapter, Index 6 (100% over 35bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT3449710.5549154902850016Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG3423250.5506591719646382TruSeq Adapter, Index 6 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG3376030.5430634292931504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC3327460.5352505275236851No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC3201350.5149646506007433No Hit
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG3080070.4954557206727885Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC2955680.47544652052652947TruSeq Adapter, Index 6 (100% over 35bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC2871250.46186522968041116No Hit
GTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC2834520.455956893629506TruSeq Adapter, Index 6 (100% over 36bp)
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC2823960.45425822690754686TruSeq Adapter, Index 6 (100% over 35bp)
GTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTCT2822870.45408289104325367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT2785320.44804265094765094Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA2777640.44680725696804424Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA2709750.4358865672186309Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2575310.414260738231871Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCA2571930.41371703619397115Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA2442100.3928327653121575Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG2423480.3898375783459757Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2258370.36327821637034396Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA2256020.36290019868127166Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2226680.35818060761855564Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG2224600.3578460217490788Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1935860.3113997121564199No Hit
TTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1839070.2958302091243722Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC1794930.28872991091345596No Hit
TCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTCCAG1784630.2870730674251814Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1690190.27188158208220603Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1673470.26919202643910406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT1660330.26707834455212087Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC1618700.26038180140485206TruSeq Adapter, Index 6 (100% over 36bp)
TGTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTC1606450.25841128366394306No Hit
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG1597900.2570359427100841Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT1575970.2535083138073792Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTCT1541940.24803429595242946Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1509540.24282247760096398TruSeq Adapter, Index 6 (100% over 37bp)
GCGCAATGGATAGCGCATTGGAGATCGGAAGAGCACACGTCTGAACTCCA1504110.24194901545132025Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG1489630.2396197830522702No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1482620.23849216432869697No Hit
ATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1386210.2229837875612652Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1353780.21776714345206685No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA1263510.20324643843395604Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCGGCTCGAAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1219730.19620404931583382Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCAG1141230.1835766499149066Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC1134300.18246189987862094TruSeq Adapter, Index 6 (100% over 36bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT1128540.18153535439391597No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG1108140.17825383913558582Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1069580.1720511318629775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1055810.16983610906360466Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGTC1038760.16709347008544148No Hit
TAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1026690.16515190689092948TruSeq Adapter, Index 6 (100% over 35bp)
ATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACT1011810.16275833105544163Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC992340.1596264142868295Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC977240.15719744956130083TruSeq Adapter, Index 6 (100% over 35bp)
CAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC967400.15561460102492983TruSeq Adapter, Index 6 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC959200.15429556057795396No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT953340.15335292923413954Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGTTGGCCCCGGATAGCCGGTTCCCCGAGATCGGAAGAGCACACGTC925500.14887462605806548No Hit
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC918080.14768105531214343TruSeq Adapter, Index 6 (100% over 35bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC907600.14599525727747187No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT899630.14471321430644782No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC881440.14178719653443678Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAACTCCAG878410.14129979500342008Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC873450.1405019363915908No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA860970.13849442117473001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC831460.13374748415152796Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC803320.1292209233981255TruSeq Adapter, Index 6 (100% over 36bp)
GTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA780250.12550991570157277Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCGGCTCGAAGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA778780.12527345357266367Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA772570.12427452172196612Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCTGAACTCCAG766520.12330132724584371Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGCAGCGGAAGCGTGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCA758140.12195333225247085Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA751070.12081606201343191TruSeq Adapter, Index 6 (100% over 37bp)
GTCTAGCGGTTAGGATTCCTAGATCGGAAGAGCACACGTCTGAACTCCAG746100.12001659481569169Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG741060.11920586751657483Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA730320.11747824624821868Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT727700.11705679673955079Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC724770.11658548106764358Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCAATGGATAGCGCATTGAGATCGGAAGAGCACACGTCTGAACTCCAG718650.11560102649014453Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA708820.11401978653968446TruSeq Adapter, Index 6 (100% over 37bp)
AGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA687960.1106642763294508Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA678180.10909107930854547Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT674600.10851520555242677TruSeq Adapter, Index 6 (100% over 50bp)
TTAGTATAGTGGTTAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACT658510.10592699082171443Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCGAACGTCTGCCCTATCAGATCGGAAGAGCACACGTCTGAACTCCAG654050.10520956150543244Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCC649980.10455486703967735Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA642380.1033323417473583Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAAC642370.10333073316144735Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAG635110.10216289979010046Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT624730.10049318761453836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)